Secretory leukoprotease inhibitor (SLPI); also called antileukoprotease (ALP); is a 12-kDa; nonglycosylated serine protease inhibitor present in mucous secretions. It is thought to play a role in protecting the mucosae from injury associated with inflammation. SLPI is locally produced by serous cells; including bronchial submucosal glands. Elafin and SLPI are members of larger families of proteins secreted predominantly at mucosal sites; and have been shown to be modulated in multiple pathological conditions. Elafin and SLPI are structurally related in that both have a fold with a four-disulfide core or whey acidic protein (WAP) domain responsible for inhibiting proteases. SLPI is a prominent innate immune protein of the respiratory tract; possessing serine protease inhibitor activity; antibacterial activity; and anti-inflammatory/immunomodulatory activity.
Product Name:
Human SLPI Recombinant Protein (RPES4528)
Product Code:
RPES4528
Size:
100µg
Species:
Human
Expressed Host:
Baculovirus-Insect Cells
Synonyms:
ALK1,ALP,BLPI,HUSI,HUSI-I,MPI,WAP4,WFDC4
Accession:
P03973
Sequence:
Ser 26-Ala 132
Fusion tag:
N-His
Activity:
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate; Mca-RPKPVE-Nval-WRK (Dnp)-NH2 (Catalog # ES002).The IC50 value is <4 Nm.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the secreted form of human SLPI (P03973) (Ser 26-Ala 132) was expressed; with a polyhistidine tag at the N-terminus.
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
