S100A16 is a member of S100 protein superfamily that carries calcium-binding EF-handmotifs. S100 proteins are cell-and tissue-specific and are involved in many intra-and extracellular processes hrough interacting with specific target proteins. S100A16 expression was found to be astrocyte-specific. The S100A16 protein was found to accumulate within nucleoli and to translocate to the cytoplasm in response to Ca(2+) stimulation. The homodimeric structure of human S100A16 in the apo state has been obtained both in the solid state and insolution; resulting in good agreement between the structures with the exception of two loop regions. The homodimeric solution structure of human S100A16 was also calculated in the calcium(II)-bound form. Immunoprecipitation analysis revealed that S100A16 could physically interact with tumor suppress or protein p53; also a known inhibitor of adipogenesis. Overexpression or RNA interference-initiated reduction of S100A16 led to the inhibition or activation of the expression of p53-responsivegenes; respectively. S100A16 protein is a novel adipogenesis-promoting factor.
Product Name:
Human S100A16/S100F Recombinant Protein (RPES0210)
Product Code:
RPES0210
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
SLAM Family Member 6, Activating NK Receptor, NK-T-B-Antigen, NTB-A, CD352, SLAMF6, KALI, Ly108, NTBA, SF2000
Accession:
Q96FQ6
Sequence:
Met1-Ser103
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human S100A16 is produced by our E.coli expression system and the target gene encoding Met1-Ser103 is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
11.8 kDa
AP Mol Mass:
22 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM Tris; 500mM NaCl; pH8.0.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
