Nucleoside-Diphosphate Kinases (NDKs) are enzymes that catalyze the exchange of phosphate groups between different nucleoside diphosphates. NDKs Possesse nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3-5 exonuclease activities. NDKs involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression and required for neural development including neural patterning and cell fate determination. Prokaryotic NDK forms a functional homotetramer.There are two isoforms of NDK in humans: NDK-A and NDK-B. Both have very similar structure, and can combine in any proportion to form functional NDK hexamers.
Product Name:
Human NME1/NDKA Recombinant Protein (RPES0826)
Product Code:
RPES0826
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Nucleoside Diphosphate Kinase A, NDK A, NDP Kinase A, Granzyme A-Activated DNase, GAAD, Metastasis Inhibition Factor nm23, Tumor Metastatic Process-Associated Protein, nm23-H1, NME1, NDPKA, NM23,AWD,GAAD,NB,NBS,NDKA,NDPK-A,NM23-H1
Accession:
P15531
Sequence:
Met 1-Glu152
Fusion tag:
N-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Nucleoside Diphosphate Kinase A is produced by our E.coli expression system and the target gene encoding Met1-Glu152 is expressed with a 6His tag at the N-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
19.3 kDa
AP Mol Mass:
20 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 20mM TrisHCl, 1mM DTT, 10% Glycerol, pH 7.5.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
