Human MGMT Recombinant Protein (RPES1838)

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SKU:
RPES1838
Product type:
Recombinant Protein
Host species:
E.coli
Reactivity:
Human
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Description

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Human MGMT Recombinant Protein

MGMT belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. MGMT involved in the cellular defense against the biological effects of O6-methylguanine in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. MGMT catalyzes the chemical reaction: DNA (containing 6-O-methylguanine) and proteinL-cysteine into DNA (without 6-O-methylguanine) and protein S-methyl-L-cysteine.

Product Name:Human MGMT Recombinant Protein (RPES1838)
Product Code:RPES1838
Size:10µg
Species:Human
Expressed Host:E.coli
Synonyms: Methylated-DNA--protein-cysteine methyltransferase, 6-O-methylguanine-DNAmethyltransferase,O-6-methylguanine-DNA-alkyltransferase, MGMT
Accession:P16455
Sequence:Met 1-Asn207
Fusion tag:N-6His
Endotoxin:<1.0 EU per µg as determined by the LAL method.
Protein Construction:Recombinant Human Methylated-DNA--protein-cysteine methyltransferase is produced by our E.coli expression system and the target gene encoding Met1-Asn207 is expressed with a 6His tag at the N-terminus.
Purity:> 90 % as determined by reducing SDS-PAGE.
Mol Mass:23.8 kDa
AP Mol Mass:25 kDa
Formulation:Supplied as a 0.2 µm filtered solution of 20mMTrisHCl,1mM EDTA, pH 8.0 .
Shipping:This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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