Glypican-5 (GPC5), is a cell membrane protein which belongs to the glypican family. The glypicans compose a family of glycosylphosphatidylinositol-anchored heparan sulfate proteoglycans that may play a role in the control of cell division and growth regulation. So far, six members (Glypican-1/GPC1, Glypican-2/GPC2, Glypican-3/GPC3, Glypican-4/GPC4, Glypican-5/GPC5, Glypican-6/GPC6) of this family are known in vertebrates. In adult, Glypican-5 is primarily expressed in the brain. It is also detected in fetal brain, lung and liver. Glypican-5 enhances the intracellular signaling of FGF2 and HGF. It alters the cellular distribution of FGF2. The properties of Glypican-5 make it an attractive target for therapeutic intervention in rhabdomyosarcomas and other tumors that amplify and/or overexpress its gene. Glypican-5 is over-expressed in lymphoma cell lines that had shown amplification. It is a likely target for amplification, and that over-expression of GPC5 may contribute to development and/or progression of lymphomas and other tumors.
Product Name:
Human Glypican 5/GPC5 Recombinant Protein (RPES2283)
Product Code:
RPES2283
Size:
50µg
Species:
Human
Expressed Host:
Baculovirus-Insect Cells
Synonyms:
GPC5,bA93M14.1,glypican proteoglycan 5,glypican-5
Accession:
NP_004457.1
Sequence:
Met 1-Thr 554
Fusion tag:
C-His
Activity:
Measured by its binding ability in a functional ELISA. Immobilized human GPC5 at 5 µg/ml (100 ?l/well) can bind human bFGF with a linear ranger of 0.156-2.5 ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human GPC5 (NP_004457.1) (Met 1-Thr 554) was fused with a polyhistidine tag at the C-terminus.
Purity:
> 92 % as determined by reducing SDS-PAGE.
Mol Mass:
60.5 kDa
AP Mol Mass:
60.5 kDa
Formulation:
Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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