Ciliary Neurotrophic Factor (CNTF) is a potent survival factor for neurons and oligodendrocytes. CNTF has also been shown to prevent the degeneration of motor axons after axotomy. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL6, IL11, LIF and OSM. All of these four helix bundle cytokines share gp130 as a signal transducing subunit in their receptor complexes. CNTF, like FGF acidic, FGF basic, and PD-ECGF (platelet-derived endothelial cell growth factor), does not possess a signal sequence that would allow secretion of the factor by classical secretion pathways. The mechanism underlying the release of CNTF is unknown.
Product Name:
Human CNTF Recombinant Protein (RPES3472)
Product Code:
RPES3472
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Ciliary Neurotrophic Factor, CNTF
Accession:
P26441
Sequence:
Ala2-Met200
Activity:
Measured in a cell proliferation assay using TF?1 human erythroleukemic cells. The ED50 for this effect is 25 ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Ciliary Neurotrophic Factor is produced by our E.coli expression system and the target gene encoding Ala2-Met200 is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
22.93 kDa
AP Mol Mass:
25 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS,1mM Cysteine,PH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
