Interferon-alpha/beta receptor beta chain (IFNAR2) is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. Initial cell-surface IFNAR2 expression at diagnosis assessed by flow cytometry widely distributed but showed overall significantly higher expression in CML patients when compared with normal controls. In 15 fresh patients who subsequently received IFNα therapy, IFNAR2 expression at diagnosis was significantly higher in cytogenetic good responders than in poor responders. Down-regulation of IFNAR2 expression during IFNα therapy was observed only in good responders but not in poor responders. The encoded protein also functions as an antiviral factor. IFNAR2 may associate with IFNAR1 to form the type I interferon receptor. This protein serves as a receptor for interferons alpha and beta. IFNAR2 is also involved in IFN-mediated STAT1, STAT2 and STAT3 activation. Isoform 1 and isoform 2 are directly involved in signal transduction due to their association with the TYR kinase, JAK1. Isoform 3 is a potent inhibitor of type I IFN receptor activity. Following binding of IFNα2, IFNAR2 is internalized, but, instead of being routed towards degradation as it is when complexed to IFNβ, it recycles back to the cell surface.
Product Name:
Human CD32a/FCGR2A Recombinant Protein (RPES3774)
Product Code:
RPES3774
Size:
50µg
Species:
Human
Expressed Host:
Baculovirus-Insect Cells
Synonyms:
Low affinity immunoglobulin gamma Fc region receptor II-a, IgG Fc receptor II-a, CDw32, Fc-gamma RII-a, Fc-gamma-RIIa, FcRII-a, CD32, FCGR2A, FCG2, FCGR2A1,IGFR2,CD32A,CDw32,Fc gamma RIIA,FCG2,FcGR,FCGR6
Accession:
AAH19931.1
Sequence:
Met 1-Gly 216
Fusion tag:
C-His
Activity:
Measured by its binding ability in a functional ELISA. Immobilized human CD32a at 10 µg/ml (100 ?l/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.07-0.17 µg/ml.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain (Met 1-Gly 216) of human CD32a (AAH19931.1) precursor was expressed, with a C-terminal polyhistidine tag.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
22 kDa
AP Mol Mass:
25 kDa
Formulation:
Lyophilized from sterile 20mM Tris, 50mM NaCl, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
system_update_alt데이터시트CD32a/FCGR2A131H Recombinant Protein Human Fc?Rs are divided into three classes designated Fc?RI (CD64); Fc?RII (CD32); and Fc?RIII (CD16); which generate multiple isoforms;