CSK Antibody (PACO62139)
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주문- SKU:
- PACO62139
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | CSK Antibody (PACO62139) |
Antibody SKU: | PACO62139 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:20-1:200 |
Species Reactivity: | Human, Mouse |
Immunogen: | Recombinant Human Tyrosine-protein kinase CSK protein (325-416AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | Antigen Affinity Purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, Raw264.7 whole cell lysate, HL-60 whole cell lysate, 293T whole cell lysate. All lanes: CSK antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 51 kDa. Observed band size: 51 kDa. | |
IHC image of PACO62139 diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK. |
Synonyms: | Tyrosine-protein kinase CSK (EC 2.7.10.2) (C-Src kinase) (Protein-tyrosine kinase CYL), CSK |
UniProt Protein Function: | CSK: a tyrosine kinase of the Csk family that phosphorylates and inhibits Src family kinases. Specifically phosphorylates Y504 on LCK, which acts as a negative regulatory site. Csk and the phospho-tyrosine phosphatase CD45 reciprocally regulate phosphorylation of the inhibitory tyrosine of the Src family kinases Lck and Fyn during T-cell receptor mediated signaling during thymic development. |
UniProt Protein Details: | Protein type:Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Protein kinase, TK; Kinase, protein; TK group; Csk family Chromosomal Location of Human Ortholog: 15q24.1 Cellular Component: cytoplasm; cytosol; extrinsic to internal side of plasma membrane; plasma membrane Molecular Function:identical protein binding; kinase activity; non-membrane spanning protein tyrosine kinase activity; protein binding; protein C-terminus binding; protein-tyrosine kinase activity; receptor binding Biological Process: cell differentiation; cell migration; central nervous system development; epidermal growth factor receptor signaling pathway; innate immune response; negative regulation of Golgi to plasma membrane protein transport; protein amino acid phosphorylation; regulation of cell proliferation; regulation of cytokine production; T cell costimulation; T cell receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway |
UniProt Code: | P41240 |
NCBI GenInfo Identifier: | 729887 |
NCBI Gene ID: | 1445 |
NCBI Accession: | P41240.1 |
UniProt Secondary Accession: | P41240,Q2M3N2, Q6FGZ6, |
UniProt Related Accession: | P41240 |
Molecular Weight: | 50,704 Da |
NCBI Full Name: | Tyrosine-protein kinase CSK |
NCBI Synonym Full Names: | c-src tyrosine kinase |
NCBI Official Symbol: | CSK |
NCBI Protein Information: | tyrosine-protein kinase CSK |
UniProt Protein Name: | Tyrosine-protein kinase CSK |
UniProt Synonym Protein Names: | C-Src kinase; Protein-tyrosine kinase CYL |
Protein Family: | Chloroplast sensor kinase |
UniProt Gene Name: | CSK |
UniProt Entry Name: | CSK_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |