CDK5RAP3 Antibody (PACO63459)
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주문- SKU:
- PACO63459
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | CDK5RAP3 Antibody (PACO63459) |
Antibody SKU: | PACO63459 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:2000, IF:1:50-1:200 |
Species Reactivity: | Human, Rat |
Immunogen: | Recombinant Human CDK5 regulatory subunit-associated protein 3 protein (42-292AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | Antigen Affinity Purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: U87 whole cell lysate, Jurkat whole cell lysate, Rat liver tissue. All lanes: CDK5RAP3 antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 57, 47, 32, 60 kDa. Observed band size: 57 kDa. | |
Immunofluorescence staining of HepG2 cells with PACO63459 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Background: | Probable tumor suppressor initially identified as a CDK5R1 interactor controlling cell proliferation. Negatively regulates NF-kappa-B-mediated gene transcription through the control of RELA phosphorylation. Also regulates mitotic G2/M transition checkpoint and mitotic G2 DNA damage checkpoint. Through its interaction with CDKN2A/ARF and MDM2 may induce MDM2-dependent p53/TP53 ubiquitination, stabilization and activation in the nucleus, thereby promoting G1 cell cycle arrest and inhibition of cell proliferation. May play a role in the unfolded protein response, mediating the ufmylation of multiple proteins in response to endoplasmic reticulum stress. May also play a role in the rupture of the nuclear envelope during apoptosis. May regulate MAPK14 activity by regulating its dephosphorylation by PPM1D/WIP1. |
Synonyms: | CDK5 regulatory subunit-associated protein 3 (CDK5 activator-binding protein C53) (LXXLL/leucine-zipper-containing ARF-binding protein) (Protein HSF-27), CDK5RAP3, IC53 LZAP |
UniProt Protein Function: | CDK5RAP3: Potential regulator of CDK5 activity. May be involved in cell proliferation. Regulates CDK5 activity via its interaction with CDK5R1. Belongs to the CDK5RAP3 family. 3 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 17q21.32 Cellular Component: protein complex; membrane; cytoplasm; nucleolus; endomembrane system; nucleus Molecular Function:cyclin binding; protein binding; protein kinase binding Biological Process: cell proliferation; regulation of neuron differentiation; positive regulation of protein ubiquitination; inhibition of NF-kappaB transcription factor; regulation of mitotic cell cycle; positive regulation of transcription from RNA polymerase II promoter; brain development; regulation of cyclin-dependent protein kinase activity |
NCBI Summary: | This gene encodes a protein that has been reported to function in signaling pathways governing transcriptional regulation and cell cycle progression. It may play a role in tumorigenesis and metastasis. A pseudogene of this gene is located on the long arm of chromosome 20. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, May 2013] |
UniProt Code: | Q96JB5 |
NCBI GenInfo Identifier: | 32129444 |
NCBI Gene ID: | 80279 |
NCBI Accession: | Q96JB5.2 |
UniProt Secondary Accession: | Q96JB5,Q53FA2, Q9H3F8, Q9H8G0, Q9HBR9, B7Z6N4, D3DTU1 D3DTU2, F5H3I5, |
UniProt Related Accession: | Q96JB5 |
Molecular Weight: | 506 |
NCBI Full Name: | CDK5 regulatory subunit-associated protein 3 |
NCBI Synonym Full Names: | CDK5 regulatory subunit associated protein 3 |
NCBI Official Symbol: | CDK5RAP3 |
NCBI Official Synonym Symbols: | C53; IC53; LZAP; HSF-27; MST016; PP1553; OK/SW-cl.114 |
NCBI Protein Information: | CDK5 regulatory subunit-associated protein 3; CDK5 regulatory subunit associated protein IC53-2; ischemic heart CDK5 activator-binding protein C53; LXXLL/leucine-zipper-containing ARFbinding protein |
UniProt Protein Name: | CDK5 regulatory subunit-associated protein 3 |
UniProt Synonym Protein Names: | CDK5 activator-binding protein C53; Protein HSF-27 |
Protein Family: | CDK5RAP3-like protein |
UniProt Gene Name: | CDK5RAP3 |
UniProt Entry Name: | CK5P3_HUMAN |
Antibodies |
Anti-CDK5RAP3 Antibody (CAB13128) |
Anti-CDK5RAP3 Antibody (CAB5000) |
CDK5RAP3 Antibody (PACO00941) |
CDK5RAP3 Antibody (PACO08341) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
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Anti-HRP-conjugated Beta Actin Antibody (CABC028) |