ATG14 Antibody (PACO50662)
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주문- SKU:
- PACO50662
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | ATG14 Antibody (PACO50662) |
Antibody SKU: | PACO50662 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:200-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
Species Reactivity: | Human, Mouse, Rat |
Immunogen: | Recombinant Human Beclin 1-associated autophagy-related key regulator protein (1-492AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Immunohistochemistry of paraffin-embedded human pancreatic cancer using PACO50662 at dilution of 1:100. | |
Western Blot. Positive WB detected in: Rat brain tissue, Mouse heart tissue, Mouse brain tissue. All lanes: ATG14 antibody at 2µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 56, 43 kDa. Observed band size: 56 kDa. | |
Immunofluorescent analysis of PC-3 cells using PACO50662 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Background: | Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1. Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK. Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion. Modulates the hepatic lipid metabolism (By similarity). |
Synonyms: | Beclin 1-associated autophagy-related key regulator (Barkor) (Autophagy-related protein 14-like protein) (Atg14L), ATG14, ATG14L KIAA0831 |
UniProt Protein Function: | Barkor: Required for both basal and inducible autophagy. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner. Forms a complex with BECN1, PIK3C3 and PIK3R4, but not with UVRAG, nor with KIAA0226/Rubicon. UVRAG and ATG14/Barkor form mutually exclusive complexes with BECN1 through direct competition. The complex containing ATG14 up-regulates autophagy, while the one containing Rubicon down-regulates autophagy. Interacts with PIK3CB. Belongs to the Barkor family. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Autophagy Chromosomal Location of Human Ortholog: 14q22.3 Cellular Component: autophagic vacuole; axoneme; cytoplasm; cytosol; endoplasmic reticulum membrane; nucleus; pre-autophagosomal structure membrane Molecular Function:protein binding Biological Process: autophagic vacuole formation; cellular response to glucose starvation; cellular response to starvation; endosome to lysosome transport; macroautophagy; negative regulation of protein amino acid phosphorylation; positive regulation of phosphoinositide 3-kinase activity; positive regulation of protein amino acid phosphorylation; regulation of protein amino acid phosphorylation |
UniProt Code: | Q6ZNE5 |
NCBI GenInfo Identifier: | 67461020 |
NCBI Gene ID: | 22863 |
NCBI Accession: | Q6ZNE5.1 |
UniProt Secondary Accession: | Q6ZNE5,O94920, Q32MK7, Q32MK8, A6NJE4, A8K9U5, B7ZWP5 |
UniProt Related Accession: | Q6ZNE5 |
Molecular Weight: | 42,434 Da |
NCBI Full Name: | Beclin 1-associated autophagy-related key regulator |
NCBI Synonym Full Names: | autophagy related 14 |
NCBI Official Symbol: | ATG14 |
NCBI Official Synonym Symbols: | ATG14L; BARKOR; KIAA0831 |
NCBI Protein Information: | beclin 1-associated autophagy-related key regulator |
UniProt Protein Name: | Beclin 1-associated autophagy-related key regulator |
UniProt Synonym Protein Names: | Autophagy-related protein 14-like protein |
Protein Family: | Autophagy-related protein |
UniProt Gene Name: | ATG14 |
UniProt Entry Name: | BAKOR_HUMAN |