Anti-SAE1 Antibody (RACO0518)
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주문- SKU:
- RACO0518
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- IF
- Application:
- ELISA
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Product Name: | SAE1 Antibody |
Product SKU: | RACO0518 |
Size: | 50ul |
Host Species: | Homo sapiens (Human) |
Tested Applications: | ELISA, WB, IF |
Recommended Dilutions: | WB:1:500-1:5000, IF:1:20-1:200 |
Species Reactivity: | Human |
Immunogen: | A synthesized peptide derived from human SAE1 |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. |
Synonyms: | SUMO-activating enzyme subunit 1 (Ubiquitin-like 1-activating enzyme E1A) [Cleaved into: SUMO-activating enzyme subunit 1, N-terminally processed], SAE1, AOS1 SUA1 UBLE1A |
Immunofluorescence staining of Hela Cells with RACO0518 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). | |
Western Blot. Positive WB detected in K562 whole cell lysate All lanes SAE1 Antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 39, 30, 34 kDa. Observed band size: 39 kDa. |
UniProt Protein Function: | UBLE1A: The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. Belongs to the ubiquitin-activating E1 family. |
UniProt Protein Details: | Protein type:Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.19 Chromosomal Location of Human Ortholog: 19q13.32 Cellular Component: cytosol; nucleoplasm; nucleus Molecular Function:ATP-dependent protein binding; enzyme activator activity; protein binding; protein C-terminus binding; protein heterodimerization activity; SUMO activating enzyme activity; ubiquitin activating enzyme activity Biological Process: protein sumoylation; protein ubiquitination; regulation of mitotic cell cycle |
NCBI Summary: | Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010] |
UniProt Code: | Q9UBE0 |
NCBI GenInfo Identifier: | 42559897 |
NCBI Gene ID: | 10055 |
NCBI Accession: | Q9UBE0.1 |
UniProt Secondary Accession: | Q9UBE0,O95717, Q9P020, B2RDP5, B3KMQ2, F5GXX7, G3XAK6 |
UniProt Related Accession: | Q9UBE0 |
Molecular Weight: | 33,063 Da |
NCBI Full Name: | SUMO-activating enzyme subunit 1 |
NCBI Synonym Full Names: | SUMO1 activating enzyme subunit 1 |
NCBI Official Symbol: | SAE1Â Â |
NCBI Official Synonym Symbols: | AOS1; SUA1; UBLE1A; HSPC140Â Â |
NCBI Protein Information: | SUMO-activating enzyme subunit 1 |
UniProt Protein Name: | SUMO-activating enzyme subunit 1 |
UniProt Synonym Protein Names: | Ubiquitin-like 1-activating enzyme E1A |
Protein Family: | SUMO-activating enzyme |
UniProt Gene Name: | SAE1Â Â |
UniProt Entry Name: | SAE1_HUMAN |