SAE1 Antibody (PACO23704)
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주문- SKU:
- PACO23704
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | SAE1 Antibody (PACO23704) |
Antibody SKU: | PACO23704 |
Size: | 100ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:3000, IF:1:100-1:500 |
Species Reactivity: | Human, Mouse, Rat |
Immunogen: | Synthesized peptide derived from Internal of human AOS1. |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Immunofluorescence analysis of HuvEc cells, using AOS1 antibody. | |
Western blot analysis of extracts from Colo cells, HepG2 cells and 293 cells using AOS1 antibody. The lane on the left is treated with synthesized peptide. | |
Western blot analysis of extracts from 293 cells, using AOS1 antibody. |
Background: | The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. |
Synonyms: | AOS1; HSPC140; SAE1; SUA1; SUMO-1 activating enzyme E1 N subunit |
UniProt Protein Function: | UBLE1A: The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. Belongs to the ubiquitin-activating E1 family. |
UniProt Protein Details: | Protein type:Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.19 Chromosomal Location of Human Ortholog: 19q13.32 Cellular Component: cytosol; nucleoplasm; nucleus Molecular Function:ATP-dependent protein binding; enzyme activator activity; protein binding; protein C-terminus binding; protein heterodimerization activity; SUMO activating enzyme activity; ubiquitin activating enzyme activity Biological Process: protein sumoylation; protein ubiquitination; regulation of mitotic cell cycle |
NCBI Summary: | Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010] |
UniProt Code: | Q9UBE0 |
NCBI GenInfo Identifier: | 42559897 |
NCBI Gene ID: | 10055 |
NCBI Accession: | Q9UBE0.1 |
UniProt Secondary Accession: | Q9UBE0,O95717, Q9P020, B2RDP5, B3KMQ2, F5GXX7, G3XAK6 |
UniProt Related Accession: | Q9UBE0 |
Molecular Weight: | 33,063 Da |
NCBI Full Name: | SUMO-activating enzyme subunit 1 |
NCBI Synonym Full Names: | SUMO1 activating enzyme subunit 1 |
NCBI Official Symbol: | SAE1 |
NCBI Official Synonym Symbols: | AOS1; SUA1; UBLE1A; HSPC140 |
NCBI Protein Information: | SUMO-activating enzyme subunit 1 |
UniProt Protein Name: | SUMO-activating enzyme subunit 1 |
UniProt Synonym Protein Names: | Ubiquitin-like 1-activating enzyme E1A |
Protein Family: | SUMO-activating enzyme |
UniProt Gene Name: | SAE1 |
UniProt Entry Name: | SAE1_HUMAN |
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