Anti-NFKBIA Antibody (RACO0174)
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주문- SKU:
- RACO0174
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- IP
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Product Name: | NFKBIA Antibody |
Product SKU: | RACO0174 |
Size: | 50ul |
Host Species: | Human |
Tested Applications: | ELISA, WB, IHC, IP |
Recommended Dilutions: | WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000 |
Species Reactivity: | Human |
Immunogen: | A synthesized peptide derived from human NFKBIA |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. |
Synonyms: | NF-kappa-B inhibitor alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, Major histocompatibility complex enhancer-binding protein MAD3, NFKBIA, IKBA, MAD3, NFKBI |
IHC image of RACO0174 diluted at 1:79.75 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunoprecipitating NFKBIA in HepG2 whole cell lysate Lane 1: Rabbit control IgG instead of RACO0174 in HepG2 whole cell lysate For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: RACO0174 (3µg) + HepG2 whole cell lysate (500µg). Lane 3: HepG2 whole cell lysate (20µg). | |
Western Blot. Positive WB detected in Hela whole cell lysate HepG2 whole cell lysate Jurkat whole cell lysate Raji whole cell lysate PC3 whole cell lysate THP-1 whole cell lysate All lanes NFKBIA antibody at 0.79µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 36 KDa. Observed band size: 36 KDa. |
UniProt Protein Function: | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. |
NCBI Summary: | This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011] |
UniProt Code: | P25963 |
NCBI GenInfo Identifier: | 126682 |
NCBI Gene ID: | 4792 |
NCBI Accession: | P25963.1 |
UniProt Secondary Accession: | P25963,B2R8L6, |
UniProt Related Accession: | P25963 |
Molecular Weight: | 35,609 Da |
NCBI Full Name: | NF-kappa-B inhibitor alpha |
NCBI Synonym Full Names: | NFKB inhibitor alpha |
NCBI Official Symbol: | NFKBIAÂ Â |
NCBI Official Synonym Symbols: | IKBA; MAD-3; NFKBIÂ Â |
NCBI Protein Information: | NF-kappa-B inhibitor alpha |
UniProt Protein Name: | NF-kappa-B inhibitor alpha |
UniProt Synonym Protein Names: | I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3 |
Protein Family: | NF-kappa-B inhibitor |
UniProt Gene Name: | NFKBIAÂ Â |
UniProt Entry Name: | IKBA_HUMAN |