Anti-NDUFS3 Antibody (RACO0378)
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주문- SKU:
- RACO0378
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Product Name: | NDUFS3 Antibody |
Product SKU: | RACO0378 |
Size: | 50ul |
Host Species: | Homo sapiens (Human) |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | WB:1:500-1:5000, IHC:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | A synthesized peptide derived from human NDUFS3 |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). |
Synonyms: | NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial (EC 1.6.5.3) (EC 1.6.99.3) (Complex I-30kD) (CI-30kD) (NADH-ubiquinone oxidoreductase 30 kDa subunit), NDUFS3 |
IHC image of RACO0378 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. | |
Western Blot. Positive WB detected in 293 whole cell lysate HepG2 whole cell lysate . All lanes NDUFS3 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 31, 15 KDa. Observed band size: 31 kDa. |
UniProt Protein Function: | NDUFS3: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I 30 kDa subunit family. |
UniProt Protein Details: | Protein type:EC 1.6.5.3; Oxidoreductase; EC 1.6.99.3; Energy Metabolism - oxidative phosphorylation; Mitochondrial Chromosomal Location of Human Ortholog: 11p11.11 Cellular Component: mitochondrial matrix; mitochondrial membrane; mitochondrial respiratory chain complex I; mitochondrion; nucleus Molecular Function:NADH dehydrogenase activity; protein binding Biological Process: mitochondrial electron transport, NADH to ubiquinone; mitochondrial respiratory chain complex I assembly; negative regulation of cell growth; substantia nigra development Disease: Leigh Syndrome; Mitochondrial Complex I Deficiency |
NCBI Summary: | This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.[provided by RefSeq, Apr 2009] |
UniProt Code: | O75489 |
NCBI GenInfo Identifier: | 6166589 |
NCBI Gene ID: | 4722 |
NCBI Accession: | O75489.1 |
UniProt Secondary Accession: | O75489,Q9UNQ8, B2R9J1, B4DFM8, |
UniProt Related Accession: | O75489 |
Molecular Weight: | 14,679 Da |
NCBI Full Name: | NADH dehydrogenase |
NCBI Synonym Full Names: | NADH:ubiquinone oxidoreductase core subunit S3 |
NCBI Official Symbol: | NDUFS3Â Â |
NCBI Official Synonym Symbols: | CI-30Â Â |
NCBI Protein Information: | NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial |
UniProt Protein Name: | NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial |
UniProt Synonym Protein Names: | Complex I-30kD; CI-30kD; NADH-ubiquinone oxidoreductase 30 kDa subunit |
UniProt Gene Name: | NDUFS3Â Â |
UniProt Entry Name: | NDUS3_HUMAN |