Anti-GFI1B Antibody (CAB16452)
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주문- SKU:
- CAB16452
- Product type:
- Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
Antibody Name: | Anti-GFI1B Antibody |
Antibody SKU: | CAB16452 |
Antibody Size: | 20uL, 50uL, 100uL |
Application: | WB |
Reactivity: | Mouse |
Host Species: | Rabbit |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human GFI1B (NP_001128503.1). |
Application: | WB |
Recommended Dilution: | WB 1:500 - 1:2000 |
Reactivity: | Mouse |
Positive Samples: |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human GFI1B (NP_001128503.1). |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | FPNQ CLDW TNLK REPE LEQD QNLA RMAP APEG PIVL SRPQ DGDS PLSD SPPF YKPS FSWD TLAT TYGH SYRQ APST MQSA FLEH SVSL YGSP LVPS TEPA L |
Gene ID: | 8328 |
Uniprot: | Q5VTD9 |
Cellular Location: | Nucleus |
Calculated MW: | 32kDa/37kDa |
Observed MW: | Refer to figures |
Synonyms: | GFI1B, BDPLT17, ZNF163B |
Background: | This gene encodes a zinc-finger containing transcriptional regulator that is primarily expressed in cells of hematopoietic lineage. The encoded protein complexes with numerous other transcriptional regulatory proteins including GATA-1, runt-related transcription factor 1 and histone deacetylases to control expression of genes involved in the development and maturation of erythrocytes and megakaryocytes. Mutations in this gene are the cause of the autosomal dominant platelet disorder, platelet-type bleeding disorder-17. Alternate splicing results in multiple transcript variants. |
UniProt Protein Function: | GFI1B: Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Component of a RCOR-GFI-KDM1A-HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation. Transcriptional repressor or activator depending on both promoter and cell type context; represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1; GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down- regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor p21Cip1/P21WAF1 in IL6-treated myelomonocytic cells. Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription; as GATA1 also mediates erythroid GFI1B transcription, both GATA1 and GFI1B participate in a feedback regulatory pathway controlling the expression of GFI1B gene in erythroid cells. Suppresses GATA1-mediated stimulation of GFI1B promoter through protein interaction. Binds to gamma- satellite DNA and to its own promoter, auto-repressing its own expression. Alters histone methylation by recruiting histone methyltransferase to target genes promoters. Plays a role in heterochromatin formation. Component of a RCOR-GFI-KDM1A-HDAC complex. Interacts directly with RCOR1, KDM1A and HDAC2. Forms a complex with GATA1. Interacts with histone methyltransferases EHMT2 and SUV39H1. Interacts with ARIH2 (via RING-type 2). Interacts with RUNX1T1. By GATA1 which binds to GFI1B promoter in cooperation with the transcription factor NFYA. Target gene of transcription factor E2-alpha/TCF3 that promotes growth arrest and apoptosis in lymphomas. Expressed in bone marrow and fetal liver, but also detectable in fetal spleen, fetal thymus, and testes. Detected in hematopoietic stem cells, erythroblasts, and megakaryocytes. Overexpressed in bone marrow of patients with erythroleukemia and megakaryocytic leukemia as well as in their corresponding leukemic cell lines, and markedly repressed in severe aplastic anemia (SAA). 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:C2H2-type zinc finger protein; Oncoprotein Chromosomal Location of Human Ortholog: 9q34.13 Cellular Component: nuclear matrix; nucleus; transcription factor complex Molecular Function:protein binding Biological Process: cell proliferation; negative regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter Disease: Bleeding Disorder, Platelet-type, 17 |
NCBI Summary: | This gene encodes a zinc-finger containing transcriptional regulator that is primarily expressed in cells of hematopoietic lineage. The encoded protein complexes with numerous other transcriptional regulatory proteins including GATA-1, runt-related transcription factor 1 and histone deacetylases to control expression of genes involved in the development and maturation of erythrocytes and megakaryocytes. Mutations in this gene are the cause of the autosomal dominant platelet disorder, platelet-type bleeding disorder-17. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014] |
UniProt Code: | Q5VTD9 |
NCBI GenInfo Identifier: | 74756792 |
NCBI Gene ID: | 8328 |
NCBI Accession: | Q5VTD9.1 |
UniProt Secondary Accession: | Q5VTD9,O95270, Q5VTD8, Q6FHZ2, Q6T888, |
UniProt Related Accession: | Q5VTD9 |
Molecular Weight: | 32,419 Da |
NCBI Full Name: | Zinc finger protein Gfi-1b |
NCBI Synonym Full Names: | growth factor independent 1B transcriptional repressor |
NCBI Official Symbol: | GFI1B |
NCBI Official Synonym Symbols: | BDPLT17; ZNF163B |
NCBI Protein Information: | zinc finger protein Gfi-1b |
UniProt Protein Name: | Zinc finger protein Gfi-1b |
UniProt Synonym Protein Names: | Growth factor independent protein 1B; Potential regulator of CDKN1A translocated in CML |
Protein Family: | Zinc finger protein |
UniProt Gene Name: | GFI1B |
UniProt Entry Name: | GFI1B_HUMAN |
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