Anti-CD97 Antibody (RACO0048)
제품 코드를 사용하여 Assay Genie 메인 사이트를 통해 주문합니다.
주문- SKU:
- RACO0048
- Product type:
- Recombinant Antibody
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- IF
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Product Name: | CD97 Antibody |
Product SKU: | RACO0048 |
Size: | 50ul |
Host Species: | Human |
Tested Applications: | ELISA, IHC, IF |
Recommended Dilutions: | IHC:1:50-1:500, IF:1:30-1:200 |
Species Reactivity: | |
Immunogen: | A synthesized peptide |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration (By similarity). |
Synonyms: | CD97 antigen, Leukocyte antigen CD97, CD97, CD97 antigen subunit alpha, CD97 antigen subunit beta, CD97 |
IHC image of RACO0048 diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunofluorescence staining of Hela cells with RACO0048 at 1:87.5,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). | |
IHC image of RACO0048 diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
UniProt Protein Function: | CD97: Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 3 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:GPCR, family 2; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 19p13.12 Cellular Component: focal adhesion; integral to plasma membrane; membrane; plasma membrane Molecular Function:protein binding; transmembrane receptor activity Biological Process: cell motility; cell-cell signaling; immune response; inflammatory response |
NCBI Summary: | This gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19. [provided by RefSeq, Jun 2011] |
UniProt Code: | P48960 |
NCBI GenInfo Identifier: | 90110013 |
NCBI Gene ID: | 976 |
NCBI Accession: | P48960.4 |
UniProt Secondary Accession: | P48960,O00718, O76101, Q8NG72, Q8TBQ7, A8K7Z4, B2RBJ9 |
UniProt Related Accession: | P48960 |
Molecular Weight: | 86,628 Da |
NCBI Full Name: | CD97 antigen |
NCBI Synonym Full Names: | adhesion G protein-coupled receptor E5 |
NCBI Official Symbol: | ADGRE5Â Â |
NCBI Official Synonym Symbols: | CD97; TM7LN1Â Â |
NCBI Protein Information: | CD97 antigen |
UniProt Protein Name: | CD97 antigen |
UniProt Synonym Protein Names: | Leukocyte antigen CD97; CD_antigen: CD97 |
Protein Family: | CD97 antigen |
UniProt Gene Name: | CD97Â Â |
UniProt Entry Name: | CD97_HUMAN |