Anti-BRD4 Antibody (RACO0215)
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주문- SKU:
- RACO0215
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Product Name: | BRD4 Antibody |
Product SKU: | RACO0215 |
Size: | 50ul |
Host Species: | Homo sapiens (Human) |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | WB:1:500-1:5000, IHC:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | A synthesized peptide derived from human Brd4 |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters: BRD4 is required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II. Promotes phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II. According to a report, directly acts as an atypical protein kinase and mediates phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II; these data however need additional evidences in vivo (PubMed:22509028). In addition to acetylated histones, also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B. Also acts as a regulator of p53/TP53-mediated transcription: following phosphorylation by CK2, recruited to p53/TP53 specific target promoters. |
Synonyms: | Bromodomain-containing protein 4 (Protein HUNK1), BRD4, HUNK1 |
IHC image of RACO0215 diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. | |
IHC image of RACO0215 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. | |
Western Blot. Positive WB detected in Hela whole cell lysate 293T whole cell lysate HepG2 whole cell lysate A549 whole cell lysate All lanes BRD4 antibody at 1:1500. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 153, 81, 89 kDa. Observed band size: 153 kDa. |
UniProt Code: | O60885 |
NCBI GenInfo Identifier: | 7657218 |
NCBI Gene ID: | 23476 |
NCBI Accession: | NP_055114.1 |
UniProt Secondary Accession: | O60885,O60433, Q4G0X8, Q86YS8, Q96PD3, |
UniProt Related Accession: | O60885 |
Molecular Weight: | 152kDa |
NCBI Full Name: | bromodomain-containing protein 4 isoform short |
NCBI Synonym Full Names: | bromodomain containing 4 |
NCBI Official Symbol: | BRD4Â Â |
NCBI Official Synonym Symbols: | CAP; MCAP; HUNK1; HUNKIÂ Â |
NCBI Protein Information: | bromodomain-containing protein 4; bromodomain-containing 4; chromosome-associated protein |
UniProt Protein Name: | Bromodomain-containing protein 4 |
UniProt Synonym Protein Names: | Protein HUNK1 |
Protein Family: | Bromodomain-containing protein |
UniProt Gene Name: | BRD4Â Â |
UniProt Entry Name: | BRD4_HUMAN |