BRD4 Antibody (PACO51358)
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주문- SKU:
- PACO51358
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | BRD4 Antibody (PACO51358) |
Antibody SKU: | PACO51358 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Bromodomain-containing protein 4 protein (150-200AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Immunofluorescent analysis of MCF-7 cells using PACO51358 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
Immunofluorescence staining of Hela cells with PACO51358 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Background: | Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters: BRD4 is required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II. Promotes phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II. According to a report, directly acts as an atypical protein kinase and mediates phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II; these data however need additional evidences in vivo. In addition to acetylated histones, also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B. Also acts as a regulator of p53/TP53-mediated transcription: following phosphorylation by CK2, recruited to p53/TP53 specific target promoters. |
Synonyms: | Bromodomain-containing protein 4 (Protein HUNK1), BRD4, HUNK1 |
UniProt Code: | O60885 |
NCBI GenInfo Identifier: | 7657218 |
NCBI Gene ID: | 23476 |
NCBI Accession: | NP_055114.1 |
UniProt Secondary Accession: | O60885,O60433, Q4G0X8, Q86YS8, Q96PD3, |
UniProt Related Accession: | O60885 |
Molecular Weight: | 152kDa |
NCBI Full Name: | bromodomain-containing protein 4 isoform short |
NCBI Synonym Full Names: | bromodomain containing 4 |
NCBI Official Symbol: | BRD4 |
NCBI Official Synonym Symbols: | CAP; MCAP; HUNK1; HUNKI |
NCBI Protein Information: | bromodomain-containing protein 4; bromodomain-containing 4; chromosome-associated protein |
UniProt Protein Name: | Bromodomain-containing protein 4 |
UniProt Synonym Protein Names: | Protein HUNK1 |
Protein Family: | Bromodomain-containing protein |
UniProt Gene Name: | BRD4 |
UniProt Entry Name: | BRD4_HUMAN |
Antibodies | ELISA Kits |
Anti-BRD4 Antibody (CAB2249) | Human BRD4 (Bromodomain-containing protein 4) ELISA Kit (HUFI05405) |
Anti-Brd4 Antibody (CAB12677) | |
Anti-Phospho-BRD4-T204 Antibody (CABP1124) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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Anti-HRP-conjugated Beta Actin Antibody (CABC028) |