Anti-Acetyl-Histone H2B type 1-B (K20) Antibody (RACO0036)
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주문- SKU:
- RACO0036
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- IF
- Application:
- ELISA
- Application:
- FC
- Application:
- ICC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Product Name: | Acetyl-Histone H2B type 1-B (K20) Antibody |
Product SKU: | RACO0036 |
Size: | 50ul |
Host Species: | Human |
Tested Applications: | ELISA, WB, ICC, IF, FC |
Recommended Dilutions: | WB:1:5000-1:10000, ICC:1:50-1:500, IF:1:30-1:200 |
Species Reactivity: | Human, Mouse |
Immunogen: | A synthesized peptide |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
Synonyms: | Histone H2B type 1-B, Histone H2B.1, Histone H2B.f, H2B/f, HIST1H2BB, H2BFF |
Immunocytochemistry analysis of RACO0036 diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunofluorescence staining of Hela cells (treated by 15mM sodium butyrate for 30min) with RACO0036 at 1:84,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). | |
Western Blot. Positive WB detected in Hela whole cell lysate treated by 15mM sodium butyrate for 30min. All lanes Acetyl-Histone H2B type 1-B (K20) antibody at 0.135µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 15 KDa. Observed band size: 15 KDa. |
UniProt Protein Function: | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
NCBI Summary: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
UniProt Code: | P33778 |
NCBI GenInfo Identifier: | 462236 |
NCBI Gene ID: | 3018 |
NCBI Accession: | P33778.2 |
UniProt Secondary Accession: | P33778,Q4KN36, |
UniProt Related Accession: | P33778 |
Molecular Weight: | 13,950 Da |
NCBI Full Name: | Histone H2B type 1-B |
NCBI Synonym Full Names: | histone cluster 1 H2B family member b |
NCBI Official Symbol: | HIST1H2BBÂ Â |
NCBI Official Synonym Symbols: | H2B.1; H2B/f; H2BFFÂ Â |
NCBI Protein Information: | histone H2B type 1-B |
UniProt Protein Name: | Histone H2B type 1-B |
UniProt Synonym Protein Names: | Histone H2B.1; Histone H2B.f; H2B/f |
Protein Family: | Histone |
UniProt Gene Name: | HIST1H2BBÂ Â |