ADRA1A Antibody (PACO61903)
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주문- SKU:
- PACO61903
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | ADRA1A Antibody (PACO61903) |
Antibody SKU: | PACO61903 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Peptide sequence from Human Alpha-1A adrenergic receptor protein (6-22AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | Antigen Affinity Purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: HepG2 whole cell lysate, K562 whole cell lysate, Hela whole cell lysate. All lanes: ADRA1A antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 52, 53, 48, 51, 33, 38, 36, 41 kDa. Observed band size: 52 kDa. | |
Immunofluorescence staining of HepG2 cells with PACO61903 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Background: | This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine(PE)-stimulated ERK signaling in cardiac myocytes. |
Synonyms: | Alpha-1A adrenergic receptor (Alpha-1A adrenoreceptor) (Alpha-1A adrenoceptor) (Alpha-1C adrenergic receptor) (Alpha-adrenergic receptor 1c), ADRA1A, ADRA1C |
UniProt Protein Function: | This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine(PE)-stimulated ERK signaling in cardiac myocytes. |
NCBI Summary: | Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1A-adrenergic receptor. Alternative splicing of this gene generates four transcript variants, which encode four different isoforms with distinct C-termini but having similar ligand binding properties. [provided by RefSeq, Jul 2008] |
UniProt Code: | P35348 |
NCBI GenInfo Identifier: | 1168246 |
NCBI Gene ID: | 148 |
NCBI Accession: | P35348.2 |
UniProt Secondary Accession: | P35348,O60451, Q13675, Q13729, A8K0I3, B0ZBD1, B0ZBD2 B0ZBD4, B0ZBD5, B0ZBD6, B0ZBD8, B0ZBD9, |
UniProt Related Accession: | P35348 |
Molecular Weight: | 40,638 Da |
NCBI Full Name: | Alpha-1A adrenergic receptor |
NCBI Synonym Full Names: | adrenoceptor alpha 1A |
NCBI Official Symbol: | ADRA1A |
NCBI Official Synonym Symbols: | ADRA1C; ADRA1L1; ALPHA1AAR |
NCBI Protein Information: | alpha-1A adrenergic receptor |
UniProt Protein Name: | Alpha-1A adrenergic receptor |
UniProt Synonym Protein Names: | Alpha-1A adrenoreceptor; Alpha-1A adrenoceptor; Alpha-1C adrenergic receptor; Alpha-adrenergic receptor 1c |
Protein Family: | Alpha-1A adrenergic receptor |
UniProt Gene Name: | ADRA1A |
UniProt Entry Name: | ADA1A_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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