ZNF703 Antibody (PACO58052)
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주문- SKU:
- PACO58052
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | ZNF703 Antibody (PACO58052) |
Antibody SKU: | PACO58052 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
Species Reactivity: | Human, Rat |
Immunogen: | Recombinant Human Zinc finger protein 703 protein (218-286AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: Rat heart tissue. All lanes: ZNF703 antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 59 kDa. Observed band size: 59 kDa. | |
Immunofluorescence staining of HepG2 cells with PACO58052 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
IHC image of PACO58052 diluted at 1:400 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | Transcriptional corepressor which does not bind directly to DNA and may regulate transcription through recruitment of histone deacetylases to gene promoters. Regulates cell adhesion, migration and proliferation. May be required for segmental gene expression during hindbrain development. |
Synonyms: | Zinc finger protein 703 (Zinc finger elbow-related proline domain protein 1), ZNF703, ZEPPO1 ZPO1 |
UniProt Protein Function: | ZNF703: Transcriptional corepressor which does not bind directly to DNA and may regulate transcription through recruitment of histone deacetylases to gene promoters. Regulates cell adhesion, migration and proliferation. May be required for segmental gene expression during hindbrain development. Luminal B breast cancers are the clinically more aggressive estrogen receptor-positive tumors. Amplification of a distal 8p12 locus occurs in around one third of the cases and ZNF703 is the single gene within the minimal amplicon. Amplification of the gene correlates with its protein expression in tumor cells. ZNF703 is a classical breast cancer oncogene since it is able to transform non-malignant cells and increase cellular proliferation. Belongs to the Elbow/Noc family. |
UniProt Protein Details: | Protein type:C2H2-type zinc finger protein Chromosomal Location of Human Ortholog: 8p11.23 Cellular Component: protein complex; nuclear matrix; cytoplasm; nucleus Molecular Function:protein binding; nucleic acid binding; metal ion binding Biological Process: regulation of transforming growth factor beta receptor signaling pathway; transcription, DNA-dependent; regulation of transcription, DNA-dependent; regulation of cell cycle; negative regulation of homotypic cell-cell adhesion; positive regulation of cell proliferation; positive regulation of mammary gland epithelial cell proliferation; negative regulation of transcription, DNA-dependent; positive regulation of cell migration |
UniProt Code: | Q9H7S9 |
NCBI GenInfo Identifier: | 74761508 |
NCBI Gene ID: | 80139 |
NCBI Accession: | Q9H7S9.1 |
UniProt Secondary Accession: | Q9H7S9,Q5XG76, |
UniProt Related Accession: | Q9H7S9 |
Molecular Weight: | 590 |
NCBI Full Name: | Zinc finger protein 703 |
NCBI Synonym Full Names: | zinc finger protein 703 |
NCBI Official Symbol: | ZNF703 |
NCBI Official Synonym Symbols: | NLZ1; ZPO1; ZEPPO1; ZNF503L |
NCBI Protein Information: | zinc finger protein 703; zinc finger elbow-related proline domain protein 1 |
UniProt Protein Name: | Zinc finger protein 703 |
UniProt Synonym Protein Names: | Zinc finger elbow-related proline domain protein 1 |
Protein Family: | Zinc finger protein |
UniProt Gene Name: | ZNF703 |
UniProt Entry Name: | ZN703_HUMAN |
Antibodies |
Anti-ZNF703 Antibody (CAB14325) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |