ZDHHC14 Antibody (PACO60212)
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주문- SKU:
- PACO60212
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | ZDHHC14 Antibody (PACO60212) |
Antibody SKU: | PACO60212 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:200-1:500 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Probable palmitoyltransferase ZDHHC14 protein (328-453AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate. All lanes: ZDHHC14 antibody at 10.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 54, 52 kDa. Observed band size: 54 kDa. | |
Immunofluorescence staining of HepG2 cells with PACO60212 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
IHC image of PACO60212 diluted at 1:600 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | endoplasmic reticulum, palmitoyltransferase activity, protein palmitoylation |
Synonyms: | Probable palmitoyltransferase ZDHHC14 (EC 2.3.1.225) (NEW1 domain-containing protein) (NEW1CP) (Zinc finger DHHC domain-containing protein 14) (DHHC-14), ZDHHC14 |
UniProt Protein Function: | ZDHHC14: Belongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:EC 2.3.1.225; Membrane protein, integral; Transferase; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 6q25.3 Cellular Component: endoplasmic reticulum Molecular Function:palmitoyltransferase activity Biological Process: protein palmitoylation |
UniProt Code: | Q8IZN3 |
NCBI GenInfo Identifier: | 37999849 |
NCBI Gene ID: | 79683 |
NCBI Accession: | Q8IZN3.1 |
UniProt Secondary Accession: | Q8IZN3,Q5JS07, Q5JS08, Q6PHS4, Q8IZN2, Q9H7F1, A6NDB7 |
UniProt Related Accession: | Q8IZN3 |
Molecular Weight: | 51,651 Da |
NCBI Full Name: | Probable palmitoyltransferase ZDHHC14 |
NCBI Synonym Full Names: | zinc finger DHHC-type containing 14 |
NCBI Official Symbol: | ZDHHC14 |
NCBI Official Synonym Symbols: | NEW1CP |
NCBI Protein Information: | probable palmitoyltransferase ZDHHC14 |
UniProt Protein Name: | Probable palmitoyltransferase ZDHHC14 |
UniProt Synonym Protein Names: | NEW1 domain-containing protein; NEW1CP; Zinc finger DHHC domain-containing protein 14; DHHC-14 |
Protein Family: | Probable palmitoyltransferase |
UniProt Gene Name: | ZDHHC14 |
UniProt Entry Name: | ZDH14_HUMAN |
Antibodies |
Anti-ZDHHC14 Antibody (CAB14538) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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