XRCC5 Antibody (PACO50102)
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주문- SKU:
- PACO50102
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IP
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | XRCC5 Antibody (PACO50102) |
Antibody SKU: | PACO50102 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IP |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human X-ray repair cross-complementing protein 5 protein (251-455AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: LO2 whole cell lysate. All lanes: XRCC5 antibody at 3.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 83 kDa. Observed band size: 83 kDa.. | |
Immunohistochemistry of paraffin-embedded human colon cancer using PACO50102 at dilution of 1:100. | |
Immunohistochemistry of paraffin-embedded human melanoma using PACO50102 at dilution of 1:100. |
Background: | Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. |
Synonyms: | X-ray repair cross-complementing protein 5 (EC 3.6.4) (86 kDa subunit of Ku antigen) (ATP-dependent DNA helicase 2 subunit 2) (ATP-dependent DNA helicase II 80 kDa subunit) (CTC box-binding factor 85 kDa subunit) (CTC85) (CTCBF) (DNA repair protein XRCC5) (Ku80) (Ku86) (Lupus Ku autoantigen protein p86) (Nuclear factor IV) (Thyroid-lupus autoantigen) (TLAA) (X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)), XRCC5, G22P2 |
UniProt Protein Function: | Ku80: the 80-kilodalton subunit of the Ku complex, also known as ATP-dependant DNA helicase II. A single stranded DNA-dependent ATP-dependent helicase. It functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by p70. |
UniProt Protein Details: | Protein type:Nucleolus; RNA-binding; DNA-binding; EC 3.6.1.-; Nuclear receptor co-regulator; Helicase; EC 3.6.4.- Chromosomal Location of Human Ortholog: 2q35 Cellular Component: cytosol; membrane; nuclear chromosome, telomeric region; nuclear telomere cap complex; nucleolus; nucleoplasm; nucleus; plasma membrane Molecular Function:5'-deoxyribose-5-phosphate lyase activity; ATP binding; ATP-dependent DNA helicase activity; damaged DNA binding; DNA binding; double-stranded DNA binding; double-stranded telomeric DNA binding; protein binding; protein C-terminus binding; telomeric DNA binding; ubiquitin protein ligase binding Biological Process: cell proliferation; DNA duplex unwinding; DNA recombination; DNA repair; double-strand break repair; double-strand break repair via nonhomologous end joining; innate immune response; negative regulation of transcription, DNA-dependent; positive regulation of interferon type I production; positive regulation of neurogenesis; regulation of smooth muscle cell proliferation; telomere maintenance; transcription, DNA-dependent; viral reproduction |
NCBI Summary: | The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008] |
UniProt Code: | P13010 |
NCBI GenInfo Identifier: | 125731 |
NCBI Gene ID: | 7520 |
NCBI Accession: | P13010.3 |
UniProt Secondary Accession: | P13010,Q0Z7V0, Q4VBQ5, Q53HH7, Q7M4N0, Q9UCQ0, Q9UCQ1 A8K3X5, |
UniProt Related Accession: | P13010 |
Molecular Weight: | 82,705 Da |
NCBI Full Name: | X-ray repair cross-complementing protein 5 |
NCBI Synonym Full Names: | X-ray repair complementing defective repair in Chinese hamster cells 5 |
NCBI Official Symbol: | XRCC5 |
NCBI Official Synonym Symbols: | KU80; KUB2; Ku86; NFIV; KARP1; KARP-1 |
NCBI Protein Information: | X-ray repair cross-complementing protein 5 |
UniProt Protein Name: | X-ray repair cross-complementing protein 5 |
UniProt Synonym Protein Names: | 86 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC box-binding factor 85 kDa subunit; CTC85; CTCBF; DNA repair protein XRCC5; Ku80; Ku86; Lupus Ku autoantigen protein p86; Nuclear factor IV; Thyroid-lupus autoantigen; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) |
UniProt Gene Name: | XRCC5 |
UniProt Entry Name: | XRCC5_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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