WTAP Antibody (PACO21850)
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주문- SKU:
- PACO21850
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | WTAP Antibody (PACO21850) |
Antibody SKU: | PACO21850 |
Size: | 100ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:3000 |
Species Reactivity: | Human, Mouse |
Immunogen: | Synthesized peptide derived from C-terminal of human WTAP. |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot analysis of extracts from HUVEC cells, using WTAP antibody. |
Background: | Regulatory subunit of the WMM N6-methyltransferase complex, a multiprotein complex that mediates N6-methyladenosine (m6A) methylation of some adenosine residues of some mRNAs and plays a role in the efficiency of mRNA splicing and processing and mRNA stability. Required for accumulation of METTL3 and METTL14 to nuclear speckle. Acts as a mRNA splicing regulator. Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes. Nagase T., DNA Res. 2:37-43(1995). |
Synonyms: | KIAA0105; Putative pre-mRNA splicing regulator female-lethal (2D) homolog; WT1-associated protein; Wilms tumor 1-associating protein; |
UniProt Protein Function: | WTAP: Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA- binding ability and inhibits expression of WT1 target genes. May be involved in mRNA splicing regulation. Belongs to the fl(2)d family. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Nucleolus; Spliceosome Chromosomal Location of Human Ortholog: 6q25-q27 Cellular Component: nuclear membrane; nuclear speck; nucleoplasm; nucleus Molecular Function:protein binding Biological Process: gene expression; regulation of alternative nuclear mRNA splicing, via spliceosome |
NCBI Summary: | The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in several transcript variants encoding three different isoforms. [provided by RefSeq, Jul 2012] |
UniProt Code: | Q15007 |
NCBI GenInfo Identifier: | 47117889 |
NCBI Gene ID: | 9589 |
NCBI Accession: | Q15007.2 |
UniProt Secondary Accession: | Q15007,Q5TCL8, Q5TCL9, Q96T28, Q9BYJ7, Q9H4E2, |
UniProt Related Accession: | Q15007 |
Molecular Weight: | 17,801 Da |
NCBI Full Name: | Pre-mRNA-splicing regulator WTAP |
NCBI Synonym Full Names: | Wilms tumor 1 associated protein |
NCBI Official Symbol: | WTAP |
NCBI Official Synonym Symbols: | Mum2 |
NCBI Protein Information: | pre-mRNA-splicing regulator WTAP |
UniProt Protein Name: | Pre-mRNA-splicing regulator WTAP |
UniProt Synonym Protein Names: | Female-lethal(2)D homolog; hFL(2)D; WT1-associated protein; Wilms tumor 1-associating protein |
Protein Family: | Pre-mRNA-splicing regulator |
UniProt Gene Name: | WTAP |
UniProt Entry Name: | FL2D_HUMAN |
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