VPS29 Antibody (PACO52870)
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주문- SKU:
- PACO52870
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | VPS29 Antibody (PACO52870) |
Antibody SKU: | PACO52870 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Vacuolar protein sorting-associated protein 29 protein (68-145AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate. All lanes: VPS29 antibody at 3.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 21 kDa. Observed band size: 28 kDa. | |
Immunofluorescent analysis of PC-3 cells using PACO52870 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using PACO52870 at dilution of 1:100. |
Background: | Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5. Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA) (Probable). Involved in GLUT1 endosome-to-plasma membrane trafficking; the function is dependent of association with ANKRD27. |
Synonyms: | Vacuolar protein sorting-associated protein 29 (hVPS29) (PEP11 homolog) (Vesicle protein sorting 29), VPS29 |
UniProt Protein Function: | VPS29: Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Has low protein phosphatase activity towards a serine-phosphorylated peptide derived from IGF2R (in vitro). Belongs to the VPS29 family. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Protein phosphatase, Ser/Thr (non-receptor); Vesicle; EC 3.1.3.3 Chromosomal Location of Human Ortholog: 12q24 Cellular Component: intracellular membrane-bound organelle; retromer complex; cytoplasm; late endosome; early endosome; endosome membrane; cytosol; endosome Molecular Function:protein binding; zinc ion binding; protein transporter activity Biological Process: intracellular protein transport; retrograde transport, endosome to Golgi |
NCBI Summary: | This gene belongs to a group of vacuolar protein sorting (VPS) genes that, when functionally impaired, disrupt the efficient delivery of vacuolar hydrolases. The protein encoded by this gene is a component of a large multimeric complex, termed the retromer complex, which is involved in retrograde transport of proteins from endosomes to the trans-Golgi network. This VPS protein may be involved in the formation of the inner shell of the retromer coat for retrograde vesicles leaving the prevacuolar compartment. Alternative splice variants encoding different isoforms and representing non-protein coding transcripts have been found for this gene. [provided by RefSeq, Aug 2013] |
UniProt Code: | Q9UBQ0 |
NCBI GenInfo Identifier: | 25453325 |
NCBI Gene ID: | 51699 |
NCBI Accession: | Q9UBQ0.1 |
UniProt Secondary Accession: | Q9UBQ0,Q502Y5, Q6FIF8, Q6IAH3, Q9H0W0, Q9NRP1, Q9NRU7 |
UniProt Related Accession: | Q9UBQ0 |
Molecular Weight: | 20,927 Da |
NCBI Full Name: | Vacuolar protein sorting-associated protein 29 |
NCBI Synonym Full Names: | vacuolar protein sorting 29 homolog (S. cerevisiae) |
NCBI Official Symbol: | VPS29 |
NCBI Official Synonym Symbols: | DC7; DC15; PEP11 |
NCBI Protein Information: | vacuolar protein sorting-associated protein 29; hVPS29; PEP11 homolog; x 007 protein; retromer protein; vesicle protein sorting 29; vacuolar sorting protein VPS29/PEP11 |
UniProt Protein Name: | Vacuolar protein sorting-associated protein 29 |
UniProt Synonym Protein Names: | PEP11 homolog; Vesicle protein sorting 29 |
Protein Family: | Vacuolar protein sorting-associated protein |
UniProt Gene Name: | VPS29 |
UniProt Entry Name: | VPS29_HUMAN |
Antibodies |
Anti-VPS29 Antibody (CAB13098) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |