USF2 Antibody (PACO57148)
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주문- SKU:
- PACO57148
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | USF2 Antibody (PACO57148) |
Antibody SKU: | PACO57148 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IF:1:50-1:200 |
Species Reactivity: | Human, Rat |
Immunogen: | Recombinant Human Upstream stimulatory factor 2 protein (21-162AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: Rat liver tissue. All lanes: USF2 antibody at 4.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 37, 31, 24 kDa. Observed band size: 37 kDa. | |
Immunofluorescence staining of MCF-7 cells with PACO57148 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Background: | Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters. |
Synonyms: | Upstream stimulatory factor 2 (Class B basic helix-loop-helix protein 12) (bHLHb12) (FOS-interacting protein) (FIP) (Major late transcription factor 2) (Upstream transcription factor 2), USF2, BHLHB12 |
UniProt Protein Function: | USF2: Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters. 3 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 19q13 Cellular Component: intracellular membrane-bound organelle; nucleoplasm; nucleus Molecular Function:bHLH transcription factor binding; protein binding; protein heterodimerization activity; protein homodimerization activity; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; transcription factor activity Biological Process: late viral mRNA transcription; lipid homeostasis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter by glucose; regulation of transcription from RNA polymerase II promoter by glucose; transcription from RNA polymerase II promoter |
NCBI Summary: | This gene encodes a member of the basic helix-loop-helix leucine zipper family of transcription factors. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs and is involved in regulating multiple cellular processes. [provided by RefSeq, Mar 2016] |
UniProt Code: | Q15853 |
NCBI GenInfo Identifier: | 2833271 |
NCBI Gene ID: | 7392 |
NCBI Accession: | Q15853.1 |
UniProt Secondary Accession: | Q15853,O00671, O00709, Q05750, Q07952, Q15851, Q15852 Q6FI33, Q6YI47, |
UniProt Related Accession: | Q15853 |
Molecular Weight: | 23,988 Da |
NCBI Full Name: | Upstream stimulatory factor 2 |
NCBI Synonym Full Names: | upstream transcription factor 2, c-fos interacting |
NCBI Official Symbol: | USF2 |
NCBI Official Synonym Symbols: | FIP; bHLHb12 |
NCBI Protein Information: | upstream stimulatory factor 2 |
UniProt Protein Name: | Upstream stimulatory factor 2 |
UniProt Synonym Protein Names: | Class B basic helix-loop-helix protein 12; bHLHb12; FOS-interacting protein; FIP; Major late transcription factor 2; Upstream transcription factor 2 |
Protein Family: | Upstream stimulatory factor |
UniProt Gene Name: | USF2 |
UniProt Entry Name: | USF2_HUMAN |
Antibodies | ELISA Kits |
Anti-USF2 Antibody (CAB8158) | USF2 Colorimetric Cell-Based ELISA Kit |
USF2 Antibody (PACO07058) | |
USF2 Antibody (PACO13110) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |