UBE2M Antibody (PACO61991)
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주문- SKU:
- PACO61991
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | UBE2M Antibody (PACO61991) |
Antibody SKU: | PACO61991 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:20-1:200 |
Species Reactivity: | Human |
Immunogen: | Peptide sequence from Human NEDD8-conjugating enzyme Ubc12 protein (11-28AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | Antigen Affinity Purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO61991 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
IHC image of PACO61991 diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation. |
Synonyms: | NEDD8-conjugating enzyme Ubc12 (EC 2.3.2) (NEDD8 carrier protein) (Ubiquitin-conjugating enzyme E2 M), UBE2M, UBC12 |
UniProt Protein Function: | Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation. |
NCBI Summary: | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq, Jul 2008] |
UniProt Code: | P61081 |
NCBI GenInfo Identifier: | 4507791 |
NCBI Gene ID: | 9040 |
NCBI Accession: | NP_003960.1 |
UniProt Secondary Accession: | P61081,O76069, Q8VC50, |
UniProt Related Accession: | P61081 |
Molecular Weight: | Observed: 21 kDaPredicted: 21 kDa |
NCBI Full Name: | NEDD8-conjugating enzyme Ubc12 |
NCBI Synonym Full Names: | ubiquitin conjugating enzyme E2 M |
NCBI Official Symbol: | UBE2M |
NCBI Official Synonym Symbols: | UBC12; hUbc12; UBC-RS2 |
NCBI Protein Information: | NEDD8-conjugating enzyme Ubc12 |
UniProt Protein Name: | NEDD8-conjugating enzyme Ubc12 |
UniProt Synonym Protein Names: | NEDD8 carrier protein; Ubiquitin-conjugating enzyme E2 M |
UniProt Gene Name: | UBE2M |
Antibodies |
Anti-UBE2M Antibody (CAB9007) |
Secondary Antibody |
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