TRAP1 Antibody (PACO31720)
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주문- SKU:
- PACO31720
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IF
- Application:
- IP
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | TRAP1 Antibody (PACO31720) |
Antibody SKU: | PACO31720 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IF, IP |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IF:1:50-1:200, IP:1:200-1:2000 |
Species Reactivity: | Human, Mouse |
Immunogen: | Recombinant Human Heat shock protein 75 kDa, mitochondrial protein (60-308AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Mouse heart tissue All lanes: TRAP1 antibody at 2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 81, 75 kDa Observed band size: 81 kDa . | |
Immunoprecipitating TRAP1 in Jurkat whole cell lysate Lane 1: Rabbit control IgG instead of PACO31720 in Jurkat whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: PACO31720 (8µg) + Jurkat whole cell lysate (500µg) Lane 3: Jurkat whole cell lysate (10µg) . | |
Immunofluorescent analysis of Hela cells using PACO31720 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Background: | Chaperone that expresses an ATPase activity. Involved in maintaining mitochondrial function and polarization, downstream of PINK1 and mitochondrial complex I. Is a negative regulator of mitochondrial respiration able to modulate the balance between oxidative phosphorylation and aerobic glycolysis. The impact of TRAP1 on mitochondrial respiration is probably mediated by modulation of mitochondrial SRC and inhibition of SDHA. |
Synonyms: | Heat shock protein 75 kDa, mitochondrial (HSP 75) (TNFR-associated protein 1) (Tumor necrosis factor type 1 receptor-associated protein) (TRAP-1), TRAP1, HSP75 |
UniProt Protein Function: | HSP75: Chaperone that expresses an ATPase activity. Belongs to the heat shock protein 90 family. |
UniProt Protein Details: | Protein type:Chaperone; Heat shock protein Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: nucleoplasm; membrane; mitochondrion; mitochondrial matrix; mitochondrial inner membrane; mitochondrial intermembrane space; lipid particle Molecular Function:protein binding; unfolded protein binding; protein kinase binding; tumor necrosis factor receptor binding; ATP binding Biological Process: response to stress |
NCBI Summary: | This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013] |
UniProt Code: | Q12931 |
NCBI GenInfo Identifier: | 67477458 |
NCBI Gene ID: | 10131 |
NCBI Accession: | Q12931.3 |
UniProt Related Accession: | Q12931 |
Molecular Weight: | 75kDa |
NCBI Full Name: | Heat shock protein 75 kDa, mitochondrial |
NCBI Synonym Full Names: | TNF receptor associated protein 1 |
NCBI Official Symbol: | TRAP1 |
NCBI Official Synonym Symbols: | HSP75; HSP 75; HSP90L; TRAP-1 |
NCBI Protein Information: | heat shock protein 75 kDa, mitochondrial |
UniProt Protein Name: | Heat shock protein 75 kDa, mitochondrial |
UniProt Synonym Protein Names: | TNFR-associated protein 1; Tumor necrosis factor type 1 receptor-associated protein; TRAP-1 |
UniProt Gene Name: | TRAP1 |
UniProt Entry Name: | TRAP1_HUMAN |
Antibodies |
Anti-TRAP1 Antibody (CAB2748) |
Anti-TRAP1 Antibody (CAB4378) |
Anti-TRAP1 Antibody (CAB3971) |
TRAP1 Antibody (PACO12892) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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Anti-HRP-conjugated Beta Actin Antibody (CABC028) |