TOP1MT Antibody (PACO18454)
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주문- SKU:
- PACO18454
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | TOP1MT Antibody (PACO18454) |
Antibody SKU: | PACO18454 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC |
Recommended Dilutions: | ELISA:1:1000-1:2000, IHC:1:5-1:20 |
Species Reactivity: | Human |
Immunogen: | Synthetic peptide of human TOP1MT |
Form: | Liquid |
Storage Buffer: | -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
Purification Method: | Antigen affinity purification |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using PACO18454(TOP1MT Antibody) at dilution 1/10, on the right is treated with synthetic peptide. (Original magnification: x200). | |
The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using PACO18454(TOP1MT Antibody) at dilution 1/10, on the right is treated with synthetic peptide. (Original magnification: x200). |
Background: | This gene encodes a mitochondrial DNA topoisomerase that plays a role in the modification of DNA topology. The encoded protein is a type IB topoisomerase and catalyzes the transient breaking and rejoining of DNA to relieve tension and DNA supercoiling generated in the mitochondrial genome during replication and transcription. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
Synonyms: | topoisomerase (DNA) I, mitochondrial |
UniProt Protein Function: | Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. |
NCBI Summary: | This gene encodes a mitochondrial DNA topoisomerase that plays a role in the modification of DNA topology. The encoded protein is a type IB topoisomerase and catalyzes the transient breaking and rejoining of DNA to relieve tension and DNA supercoiling generated in the mitochondrial genome during replication and transcription. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012] |
UniProt Code: | Q969P6 |
NCBI GenInfo Identifier: | 386642871 |
NCBI Gene ID: | 116447 |
NCBI Accession: | NP_001245375.1 |
UniProt Secondary Accession: | Q969P6,Q86ST4, Q86V82, B7ZAR5, E7ES89, |
UniProt Related Accession: | Q969P6 |
Molecular Weight: | 66 |
NCBI Full Name: | DNA topoisomerase I, mitochondrial isoform 2 |
NCBI Synonym Full Names: | DNA topoisomerase I mitochondrial |
NCBI Official Symbol: | TOP1MT |
NCBI Protein Information: | DNA topoisomerase I, mitochondrial |
UniProt Protein Name: | DNA topoisomerase I, mitochondrial |
UniProt Gene Name: | TOP1MT |
Antibodies |
Anti-TOP1MT Antibody (CAB15559) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |