TFEB Antibody (PACO50054)
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주문- SKU:
- PACO50054
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | TFEB Antibody (PACO50054) |
Antibody SKU: | PACO50054 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
Species Reactivity: | Human, Rat |
Immunogen: | Recombinant Human Transcription factor EB protein (1-476AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Immunohistochemistry of paraffin-embedded human placenta tissue using PACO50054 at dilution of 1:100. | |
Western Blot. Positive WB detected in: Rat lung tissue. All lanes: TFEB antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 53, 44 kDa. Observed band size: 70 kDa. | |
Immunofluorescent analysis of Hela cells using PACO50054 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Background: | Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Acts as a positive regulator of autophagy by promoting expression of genes involved in autophagy. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta. |
Synonyms: | Transcription factor EB (Class E basic helix-loop-helix protein 35) (bHLHe35), TFEB, BHLHE35 |
UniProt Protein Function: | TFEB: Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T- cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta. Homodimer and heterodimer; with TFE3 or MITF. Belongs to the MiT/TFE family. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 6p21 Cellular Component: cytoplasm; nucleus Molecular Function:transcription factor activity Biological Process: embryonic placenta development; humoral immune response; lysosome organization and biogenesis; positive regulation of autophagy; positive regulation of transcription from RNA polymerase II promoter |
UniProt Code: | P19484 |
NCBI GenInfo Identifier: | 19856774 |
NCBI Gene ID: | 7942 |
NCBI Accession: | P19484.3 |
UniProt Secondary Accession: | P19484,Q709B3, Q7Z6P9, Q9BRJ5, Q9UJD8, |
UniProt Related Accession: | P19484 |
Molecular Weight: | 43,803 Da |
NCBI Full Name: | Transcription factor EB |
NCBI Synonym Full Names: | transcription factor EB |
NCBI Official Symbol: | TFEB |
NCBI Official Synonym Symbols: | TCFEB; BHLHE35; ALPHATFEB |
NCBI Protein Information: | transcription factor EB |
UniProt Protein Name: | Transcription factor EB |
UniProt Synonym Protein Names: | Class E basic helix-loop-helix protein 35; bHLHe35 |
Protein Family: | Transcription factor |
UniProt Gene Name: | TFEB |
UniProt Entry Name: | TFEB_HUMAN |
Antibodies |
Anti-TFEB Antibody (CAB11796) |
Anti-TFEB Antibody (CAB7311) |
TFEB Antibody (PACO06990) |
TFEB Antibody (PACO12715) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |