SNAP23 Antibody (PACO55790)
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주문- SKU:
- PACO55790
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | SNAP23 Antibody (PACO55790) |
Antibody SKU: | PACO55790 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Synaptosomal-associated protein 23 protein (90-196AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO55790 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunofluorescence staining of Hela cells with PACO55790 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
IHC image of PACO55790 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated ABC system. |
Background: | Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion. |
Synonyms: | Synaptosomal-associated protein 23 (SNAP-23) (Vesicle-membrane fusion protein SNAP-23), SNAP23 |
UniProt Protein Function: | SNAP23: a member of the SNAP-25 family of SNARE proteins. Structurally and functionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs. It is an essential component of the high affinity receptor for the general membrane fusion machinery and is an important regulator of transport vesicle docking and fusion. Two alternatively spliced isoforms have been described. |
UniProt Protein Details: | Protein type:Vesicle Chromosomal Location of Human Ortholog: 15q14 Cellular Component: nucleoplasm; SNARE complex; azurophil granule; specific granule; neuron projection; mast cell granule; focal adhesion; cytoplasm; plasma membrane; synapse Molecular Function:SNAP receptor activity; protein binding; syntaxin binding Biological Process: synaptic vesicle fusion to presynaptic membrane; protein transport; histamine secretion by mast cell; vesicle targeting; post-Golgi vesicle-mediated transport; synaptic vesicle priming |
NCBI Summary: | Specificity of vesicular transport is regulated, in part, by the interaction of a vesicle-associated membrane protein termed synaptobrevin/VAMP with a target compartment membrane protein termed syntaxin. These proteins, together with SNAP25 (synaptosome-associated protein of 25 kDa), form a complex which serves as a binding site for the general membrane fusion machinery. Synaptobrevin/VAMP and syntaxin are believed to be involved in vesicular transport in most, if not all cells, while SNAP25 is present almost exclusively in the brain, suggesting that a ubiquitously expressed homolog of SNAP25 exists to facilitate transport vesicle/target membrane fusion in other tissues. The protein encoded by this gene is structurally and functionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs. It is an essential component of the high affinity receptor for the general membrane fusion machinery and is an important regulator of transport vesicle docking and fusion. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
UniProt Code: | O00161 |
NCBI GenInfo Identifier: | 6685971 |
NCBI Gene ID: | 8773 |
NCBI Accession: | O00161.1 |
UniProt Secondary Accession: | O00161,O00162, Q13602, Q6IAE3, |
UniProt Related Accession: | O00161 |
Molecular Weight: | 17,789 Da |
NCBI Full Name: | Synaptosomal-associated protein 23 |
NCBI Synonym Full Names: | synaptosomal-associated protein, 23kDa |
NCBI Official Symbol: | SNAP23 |
NCBI Official Synonym Symbols: | SNAP-23; SNAP23A; SNAP23B; HsT17016 |
NCBI Protein Information: | synaptosomal-associated protein 23; synaptosomal-associated protein, 23kD; vesicle-membrane fusion protein SNAP-23 |
UniProt Protein Name: | Synaptosomal-associated protein 23 |
UniProt Synonym Protein Names: | Vesicle-membrane fusion protein SNAP-23 |
Protein Family: | Synaptosomal-associated protein |
UniProt Gene Name: | SNAP23 |
UniProt Entry Name: | SNP23_HUMAN |
Antibodies | ELISA Kits |
Anti-SNAP23 Antibody (CAB13909) | Human SNAP23 (Synaptosomal-associated protein 23) ELISA Kit (HUFI06433) |
SNAP23 Antibody (PACO01942) | |
SNAP23 Antibody (PACO12357) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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