SMG7 Antibody (PACO59293)
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주문- SKU:
- PACO59293
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | SMG7 Antibody (PACO59293) |
Antibody SKU: | PACO59293 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:500-1:1000, IF:1:200-1:500 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Protein SMG7 protein (694-809AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO59293 diluted at 1:600 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunofluorescence staining of HepG2 cells with PACO59293 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
IHC image of PACO59293 diluted at 1:600 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | Plays a role in nonsense-mediated mRNA decay. Recruits UPF1 to cytoplasmic mRNA decay bodies. Together with SMG5 is thought to provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. |
Synonyms: | Protein SMG7 (EST1-like protein C) (SMG-7 homolog) (hSMG-7), SMG7, C1orf16 EST1C KIAA0250 |
UniProt Protein Function: | Plays a role in nonsense-mediated mRNA decay. Recruits UPF1 to cytoplasmic mRNA decay bodies. Together with SMG5 is thought to provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. |
NCBI Summary: | This gene encodes a protein that is essential for nonsense-mediated mRNA decay (NMD); a process whereby transcripts with premature termination codons are targeted for rapid degradation by a mRNA decay complex. The mRNA decay complex consists, in part, of this protein along with proteins SMG5 and UPF1. The N-terminal domain of this protein is thought to mediate its association with SMG5 or UPF1 while the C-terminal domain interacts with the mRNA decay complex. This protein may therefore couple changes in UPF1 phosphorylation state to the degradation of NMD-candidate transcripts. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Aug 2011] |
UniProt Code: | Q92540 |
NCBI GenInfo Identifier: | 84028262 |
NCBI Gene ID: | 9887 |
NCBI Accession: | Q92540.2 |
UniProt Secondary Accession: | Q92540,Q5T1Q0, Q6PIE0, Q7Z7H9, Q8IXC1, Q8IXC2, B4DRB2 E9PCI0, E9PEH2, |
UniProt Related Accession: | Q92540 |
Molecular Weight: | 127,854 Da |
NCBI Full Name: | Protein SMG7 |
NCBI Synonym Full Names: | SMG7, nonsense mediated mRNA decay factor |
NCBI Official Symbol: | SMG7 |
NCBI Official Synonym Symbols: | EST1C; SGA56M; C1orf16 |
NCBI Protein Information: | protein SMG7 |
UniProt Protein Name: | Protein SMG7 |
UniProt Synonym Protein Names: | EST1-like protein C; SMG-7 homolog; hSMG-7 |
Protein Family: | Protein |
UniProt Gene Name: | SMG7 |
UniProt Entry Name: | SMG7_HUMAN |
Antibodies | ELISA Kits |
Anti-SMG7 Antibody (CAB15371) | SMG7 Colorimetric Cell-Based ELISA |
SMG7 Antibody (PACO06816) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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