SGMS1 Antibody (PACO53874)
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주문- SKU:
- PACO53874
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | SGMS1 Antibody (PACO53874) |
Antibody SKU: | PACO53874 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Phosphatidylcholine:ceramide cholinephosphotransferase 1 protein (48-137AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: K562 whole cell lysate. All lanes: SGMS1 antibody at 4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 49, 26 kDa. Observed band size: 49 kDa. | |
IHC image of PACO53874 diluted at 1:400 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | Sphingomyelin synthases synthesize the sphingolipid, sphingomyelin, through transfer of the phosphatidyl head group, phosphatidylcholine, on to the primary hydroxyl of ceramide. The reaction is bidirectional depending on the respective levels of the sphingolipid and ceramide. Golgi apparatus SMS1 directly and specifically recognizes the choline head group on the substrate, requiring two fatty chains on the choline-P donor molecule in order to be recognized efficiently as a substrate. Major form in macrophages. Required for cell growth in certain cell types such as HeLa cells. Suppresses BAX-mediated apoptosis and also prevents cell death in response to stimuli such as hydrogen peroxide, osmotic stress, elevated temperature and exogenously supplied sphingolipids. May protect against cell death by reversing the stress-inducible increase in levels of proapoptotic ceramide. |
Synonyms: | Phosphatidylcholine:ceramide cholinephosphotransferase 1 (EC 2.7.8.27) (Medulla oblongata-derived protein) (Protein Mob) (Sphingomyelin synthase 1) (Transmembrane protein 23), SGMS1, MOB SMS1 TMEM23 |
UniProt Protein Function: | TMEM23: Sphingomyelin synthases synthesize the sphingolipid, sphingomyelin, through transfer of the phosphatidyl head group, phosphatidylcholine, on to the primary hydroxyl of ceramide. The reaction is bidirectional depending on the respective levels of the sphingolipid and ceramide. Golgi apparatus SMS1 directly and specifically recognizes the choline head group on the substrate, requiring two fatty chains on the choline-P donor molecule in order to be recognized efficiently as a substrate. Major form in macrophages. Required for cell growth in certain cell types such as HeLa cells. Suppresses BAX-mediated apoptosis and also prevents cell death in response to stimuli such as hydrogen peroxide, osmotic stress, elevated temperature and exogenously supplied sphingolipids. May protect against cell death by reversing the stress-inducible increase in levels of proapoptotic ceramide. Belongs to the sphingomyelin synthase family. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Membrane protein, multi-pass; EC 2.7.8.27; Membrane protein, integral; Lipid Metabolism - sphingolipid; Transferase Chromosomal Location of Human Ortholog: 10q11.2 Cellular Component: Golgi membrane; membrane; endoplasmic reticulum; Golgi trans cisterna; plasma membrane; integral to Golgi membrane; nucleus Molecular Function:sphingomyelin synthase activity; kinase activity; ceramide cholinephosphotransferase activity Biological Process: sphingolipid metabolic process; sphingolipid biosynthetic process; apoptosis; cell growth; sphingomyelin biosynthetic process; inflammatory response; phosphorylation |
NCBI Summary: | The protein encoded by this gene is predicted to be a five-pass transmembrane protein. This gene may be predominately expressed in brain. [provided by RefSeq, Jul 2008] |
UniProt Code: | Q86VZ5 |
NCBI GenInfo Identifier: | 44888473 |
NCBI Gene ID: | 259230 |
NCBI Accession: | Q86VZ5.2 |
UniProt Secondary Accession: | Q86VZ5,Q68U43, Q6EKK0, Q75SP1, |
UniProt Related Accession: | Q86VZ5 |
Molecular Weight: | 419 |
NCBI Full Name: | Phosphatidylcholine:ceramide cholinephosphotransferase 1 |
NCBI Synonym Full Names: | sphingomyelin synthase 1 |
NCBI Official Symbol: | SGMS1 |
NCBI Official Synonym Symbols: | MOB; MOB1; SMS1; TMEM23; hmob33 |
NCBI Protein Information: | phosphatidylcholine:ceramide cholinephosphotransferase 1; protein Mob; transmembrane protein 23; medulla oblongata-derived protein |
UniProt Protein Name: | Phosphatidylcholine:ceramide cholinephosphotransferase 1 |
UniProt Synonym Protein Names: | Medulla oblongata-derived protein; Protein Mob; Sphingomyelin synthase 1; Transmembrane protein 23 |
UniProt Gene Name: | SGMS1 |
UniProt Entry Name: | SMS1_HUMAN |