SEMA3A Antibody (PACO55878)
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주문- SKU:
- PACO55878
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | SEMA3A Antibody (PACO55878) |
Antibody SKU: | PACO55878 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500 |
Species Reactivity: | Human, Rat, Mouse |
Immunogen: | Recombinant Human Semaphorin-3A protein (357-445AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, Rat kidney tissue, Mouse heart tissue, Mouse skeletal muscle tissue. All lanes: SEMA3A antibody at 3.6µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 89 kDa. Observed band size: 89 kDa. | |
IHC image of PACO55878 diluted at 1:300 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated ABC system. | |
IHC image of PACO55878 diluted at 1:300 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | Involved in the development of the olfactory system and in neuronal control of puberty. Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1. |
Synonyms: | Semaphorin-3A (Semaphorin III) (Sema III), SEMA3A, SEMAD |
UniProt Protein Function: | SEMA3A: Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1. Belongs to the semaphorin family. |
UniProt Protein Details: | Protein type:Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide Chromosomal Location of Human Ortholog: 7p12.1 Cellular Component: axon; extracellular region; extracellular space Molecular Function:chemorepellent activity; semaphorin receptor binding Biological Process: axon extension involved in axon guidance; axon guidance; negative regulation of axon extension involved in axon guidance; nerve development; neural crest cell migration; neuron migration; olfactory bulb development; regulation of axon extension involved in axon guidance; sensory system development; sympathetic nervous system development Disease: Hypogonadotropic Hypogonadism 16 With Or Without Anosmia |
NCBI Summary: | This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease. [provided by RefSeq, Jul 2008] |
UniProt Code: | Q14563 |
NCBI GenInfo Identifier: | 8134674 |
NCBI Gene ID: | 10371 |
NCBI Accession: | Q14563.1 |
UniProt Related Accession: | Q14563 |
Molecular Weight: | |
NCBI Full Name: | Semaphorin-3A |
NCBI Synonym Full Names: | semaphorin 3A |
NCBI Official Symbol: | SEMA3A |
NCBI Official Synonym Symbols: | HH16; SemD; COLL1; SEMA1; SEMAD; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III |
NCBI Protein Information: | semaphorin-3A |
UniProt Protein Name: | Semaphorin-3A |
UniProt Synonym Protein Names: | Semaphorin III; Sema III |
Protein Family: | Semaphorin |
UniProt Gene Name: | SEMA3A |
UniProt Entry Name: | SEM3A_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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Anti-HRP-conjugated Beta Actin Antibody (CABC028) |