RUFY3 Antibody (PACO59169)
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주문- SKU:
- PACO59169
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | RUFY3 Antibody (PACO59169) |
Antibody SKU: | PACO59169 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Protein RUFY3 protein (243-352AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, SH-SY5Y whole cell lysate. All lanes: RUFY3 antibody at 3.71µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 53, 56, 71, 65 kDa. Observed band size: 53 kDa. | |
Immunofluorescence staining of Hela cells with PACO59169 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Background: | Plays a role in the generation of neuronal polarity formation and axon growth (By similarity). Implicated in the formation of a single axon by developing neurons (By similarity). May inhibit the formation of additional axons by inhibition of PI3K in minor neuronal processes (By similarity). Plays a role in the formation of F-actin-enriched protrusive structures at the cell periphery. Plays a role in cytoskeletal organization by regulating the subcellular localization of FSCN1 and DBN1 at axonal growth cones (By similarity). Promotes gastric cancer cell migration and invasion in a PAK1-dependent manner. |
Synonyms: | Protein RUFY3 (RUN and FYVE domain-containing protein 3) (Rap2-interacting protein x) (RIPx) (Single axon-regulated protein) (Singar), RUFY3, KIAA0871 |
UniProt Protein Function: | RUFY3: Implicated in the formation of a single axon by developing neurons. May inhibit the formation of additional axons by inhibition of PI3K in minor neuronal processes. 4 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Cell development/differentiation Chromosomal Location of Human Ortholog: 4q13.3 Cellular Component: axon; cytoplasm; cytosol; dendrite; filopodium; growth cone; lamellipodium; perikaryon Molecular Function:protein binding Biological Process: actin filament organization; negative regulation of axonogenesis; positive regulation of axon extension; positive regulation of cell migration |
NCBI Summary: | This gene encodes a RPIP8, UNC-14, and NESCA domain-containing protein that is required for maintenance of neuronal polarity. In addition, it has been implicated in mediation of gastric cancer cell migration and invasion via interaction with P21-activated kinase-1, which promotes its expression. The encoded protein localizes to F-actin-enriched invadopodia to induce formation of protrusions, thereby facilitating cell migration. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016] |
UniProt Code: | Q7L099 |
NCBI GenInfo Identifier: | 74738521 |
NCBI Gene ID: | 22902 |
NCBI Accession: | Q7L099.1 |
UniProt Secondary Accession: | Q7L099,O94948, Q9UI00, B3KM25, B4DYW7, D9N163, |
UniProt Related Accession: | Q7L099 |
Molecular Weight: | 53kDa |
NCBI Full Name: | Protein RUFY3 |
NCBI Synonym Full Names: | RUN and FYVE domain containing 3 |
NCBI Official Symbol: | RUFY3 |
NCBI Official Synonym Symbols: | RIPX; SINGAR1; ZFYVE30 |
NCBI Protein Information: | protein RUFY3 |
UniProt Protein Name: | Protein RUFY3 |
UniProt Synonym Protein Names: | RUN and FYVE domain-containing protein 3 |
Protein Family: | Protein |
UniProt Gene Name: | RUFY3 |
Antibodies |
Anti-RUFY3 Antibody (CAB12896) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |