RPL7A Antibody (PACO60805)
제품 코드를 사용하여 Assay Genie 메인 사이트를 통해 주문합니다.
주문- SKU:
- PACO60805
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | RPL7A Antibody (PACO60805) |
Antibody SKU: | PACO60805 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500 |
Species Reactivity: | Human, Mouse |
Immunogen: | Recombinant Human 60S ribosomal protein L7a protein (2-138AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: RAW264.7 whole cell lysate, NIH/3T3 whole cell lysate, MCF-7 whole cell lysate. All lanes: RPL7A antibody at 5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 30 kDa. Observed band size: 30 kDa. | |
IHC image of PACO60805 diluted at 1:400 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | cytoplasm, cytosol, cytosolic large ribosomal subunit, extracellular exosome, focal adhesion, membrane, nucleolus, nucleus, polysomal ribosome, cadherin binding |
Synonyms: | 60S ribosomal protein L7a (Large ribosomal subunit protein eL8) (PLA-X polypeptide) (Surfeit locus protein 3), RPL7A, SURF-3 SURF3 |
UniProt Protein Function: | RPL7A: Belongs to the ribosomal protein L7Ae family. |
UniProt Protein Details: | Protein type:Nuclear receptor co-regulator; Translation; Ribosomal Chromosomal Location of Human Ortholog: 9q34 Cellular Component: cytoplasm; cytosol; focal adhesion; membrane; nucleolus; nucleus; polysomal ribosome Molecular Function:protein binding; RNA binding Biological Process: maturation of LSU-rRNA; mRNA catabolic process, nonsense-mediated decay; rRNA processing; SRP-dependent cotranslational protein targeting to membrane; translational initiation; viral transcription |
NCBI Summary: | Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L7AE family of ribosomal proteins. It can interact with a subclass of nuclear hormone receptors, including thyroid hormone receptor, and inhibit their ability to transactivate by preventing their binding to their DNA response elements. This gene is included in the surfeit gene cluster, a group of very tightly linked genes that do not share sequence similarity. It is co-transcribed with the U24, U36a, U36b, and U36c small nucleolar RNA genes, which are located in its second, fifth, fourth, and sixth introns, respectively. This gene rearranges with the trk proto-oncogene to form the chimeric oncogene trk-2h, which encodes an oncoprotein consisting of the N terminus of ribosomal protein L7a fused to the receptor tyrosine kinase domain of trk. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008] |
UniProt Code: | P62424 |
NCBI GenInfo Identifier: | 54039239 |
NCBI Gene ID: | 6130 |
NCBI Accession: | P62424.2 |
UniProt Secondary Accession: | P62424,P11518, Q5T8U4, |
UniProt Related Accession: | P62424 |
Molecular Weight: | 29,996 Da |
NCBI Full Name: | 60S ribosomal protein L7a |
NCBI Synonym Full Names: | ribosomal protein L7a |
NCBI Official Symbol: | RPL7A |
NCBI Official Synonym Symbols: | L7A; TRUP; SURF3 |
NCBI Protein Information: | 60S ribosomal protein L7a |
UniProt Protein Name: | 60S ribosomal protein L7a |
UniProt Synonym Protein Names: | PLA-X polypeptide; Surfeit locus protein 3 |
Protein Family: | 60S ribosomal protein |
UniProt Gene Name: | RPL7A |
UniProt Entry Name: | RL7A_HUMAN |