RAP1A Antibody (PACO29372)
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주문- SKU:
- PACO29372
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | RAP1A Antibody (PACO29372) |
Antibody SKU: | PACO29372 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:500 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Ras-related protein Rap-1A protein (1-181AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using PACO29372 at dilution of 1:100. | |
Immunofluorescence staining of Hela cells with PACO29372 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
Immunohistochemistry of paraffin-embedded human pancreatic tissue using PACO29372 at dilution of 1:100. |
Background: | Induces morphological reversion of a cell line transformed by a Ras oncogene. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Together with ITGB1BP1, regulates KRIT1 localization to microtubules and membranes. Plays a role in nerve growth factor (NGF)-induced neurite outgrowth. Plays a role in the regulation of embryonic blood vessel formation. Involved in the establishment of basal endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR expression at endothelial cell-cell junctions. |
Synonyms: | Ras-related protein Rap-1A (C21KG) (G-22K) (GTP-binding protein smg p21A) (Ras-related protein Krev-1), RAP1A, KREV1 |
UniProt Protein Function: | RAP1A: a small G protein of the Ras family with tumor suppressor activity. Induces morphological reversion of a cell line transformed by a Ras oncogene. Activated by guanine nucleotide-exchange factors (GEF) EPAC and EPAC2 in a cAMP-dependent manner, and GFR. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Interacts with SGSM1, SGSM2 and SGSM3. |
UniProt Protein Details: | Protein type:Tumor suppressor; G protein, monomeric; Motility/polarity/chemotaxis; G protein; G protein, monomeric, Ras Chromosomal Location of Human Ortholog: 1p13.3 Cellular Component: neuron projection; perinuclear region of cytoplasm; late endosome; cytoplasm; early endosome; plasma membrane; cell junction; cytosol Molecular Function:GTPase activity; Rap guanyl-nucleotide exchange factor activity; protein binding; Ras GTPase binding; GTP binding; protein complex binding; protein transporter activity Biological Process: platelet activation; microvillus biogenesis; protein transport; nerve growth factor receptor signaling pathway; activation of MAPKK activity; positive regulation of protein kinase activity; energy reserve metabolic process; blood coagulation; signal transduction; regulation of insulin secretion; Rap protein signal transduction |
NCBI Summary: | This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014] |
UniProt Code: | P62834 |
NCBI GenInfo Identifier: | 51338607 |
NCBI Gene ID: | 5906 |
NCBI Accession: | P62834.1 |
UniProt Secondary Accession: | P62834,P10113, |
UniProt Related Accession: | P62834 |
Molecular Weight: | 184 |
NCBI Full Name: | Ras-related protein Rap-1A |
NCBI Synonym Full Names: | RAP1A, member of RAS oncogene family |
NCBI Official Symbol: | RAP1A |
NCBI Official Synonym Symbols: | RAP1; C21KG; G-22K; KREV1; KREV-1; SMGP21 |
NCBI Protein Information: | ras-related protein Rap-1A; Ras-related protein Krev-1; GTP-binding protein smg p21A |
UniProt Protein Name: | Ras-related protein Rap-1A |
UniProt Synonym Protein Names: | C21KG; G-22K; GTP-binding protein smg p21A; Ras-related protein Krev-1 |
Protein Family: | Ras-related protein |
UniProt Gene Name: | RAP1A |
UniProt Entry Name: | RAP1A_HUMAN |
Antibodies | ELISA Kits |
Anti-RAP1A Antibody (CAB0975) | Human RAP1A ELISA Kit |
RAP1A Antibody (PACO11715) | Human Ras-related protein Rap-1A (RAP1A) ELISA Kit |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |