RAD54L2 Antibody (PACO57608)
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주문- SKU:
- PACO57608
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | RAD54L2 Antibody (PACO57608) |
Antibody SKU: | PACO57608 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IF:1:200-1:500 |
Species Reactivity: | Human, Rat |
Immunogen: | Recombinant Human Helicase ARIP4 protein (1122-1296AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Immunofluorescence staining of Hela cells with PACO57608 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
Western Blot. Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, U87 whole cell lysate, Rat brain tissue. All lanes: RAD54L2 antibody at 3.7µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 163 kDa. Observed band size: 163 kDa. |
Background: | DNA helicase that modulates androgen receptor (AR)-dependent transactivation in a promoter-dependent manner. Not able to remodel mononucleosomes in vitro (By similarity). |
Synonyms: | Helicase ARIP4 (EC 3.6.4.12) (Androgen receptor-interacting protein 4) (RAD54-like protein 2), RAD54L2, ARIP4 KIAA0809 |
UniProt Protein Function: | RAD54L2: DNA helicase that modulates androgen receptor (AR)- dependent transactivation in a promoter-dependent manner. Not able to remodel mononucleosomes in vitro. Belongs to the SNF2/RAD54 helicase family. |
UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor; EC 3.6.4.12; Nuclear receptor co-regulator; Helicase Chromosomal Location of Human Ortholog: 3p21.2 Cellular Component: nucleus Molecular Function:DNA binding; transcription cofactor activity; protein kinase binding; helicase activity; ATP binding Biological Process: metabolic process |
UniProt Code: | Q9Y4B4 |
NCBI GenInfo Identifier: | 296439458 |
NCBI Gene ID: | 23132 |
NCBI Accession: | Q9Y4B4.4 |
UniProt Secondary Accession: | Q9Y4B4,Q8TB57, Q9BV54, |
UniProt Related Accession: | Q9Y4B4 |
Molecular Weight: | 1467 |
NCBI Full Name: | Helicase ARIP4 |
NCBI Synonym Full Names: | RAD54-like 2 (S. cerevisiae) |
NCBI Official Symbol: | RAD54L2 |
NCBI Official Synonym Symbols: | ARIP4; HSPC325; SRISNF2L |
NCBI Protein Information: | helicase ARIP4; RAD54-like protein 2; androgen receptor-interacting protein 4 |
UniProt Protein Name: | Helicase ARIP4 |
UniProt Synonym Protein Names: | Androgen receptor-interacting protein 4; RAD54-like protein 2 |
Protein Family: | Helicase |
UniProt Gene Name: | RAD54L2 |
UniProt Entry Name: | ARIP4_HUMAN |