RAB21 Antibody (PACO59557)
제품 코드를 사용하여 Assay Genie 메인 사이트를 통해 주문합니다.
주문- SKU:
- PACO59557
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | RAB21 Antibody (PACO59557) |
Antibody SKU: | PACO59557 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Ras-related protein Rab-21 protein (141-222AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO59557 diluted at 1:400 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunofluorescence staining of HepG2 cells with PACO59557 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Background: | Regulates integrin internalization and recycling, but does not influence the traffic of endosomally translocated receptors in general. As a result, may regulate cell adhesion and migration (By similarity). During the mitosis of adherent cells, controls the endosomal trafficking of integrins which is required for the successful completion of cytokinesis. Involved in neurite growth (By similarity). |
Synonyms: | Ras-related protein Rab-21, RAB21, KIAA0118 |
UniProt Protein Function: | RAB21: Regulates integrin internalization and recycling, but does not influence the traffic of endosomally translocated receptors in general. As a result, may regulate cell adhesion and migration. During the mitosis of adherent cells, controls the endosomal trafficking of integrins which is required for the successful completion of cytokinesis. Belongs to the small GTPase superfamily. Rab family. |
UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; G protein, monomeric; G protein, monomeric, Rab Chromosomal Location of Human Ortholog: 12q21.1 Cellular Component: endosome; focal adhesion; internal side of plasma membrane; trans-Golgi network; vesicle membrane Molecular Function:GDP binding; GTP binding; GTPase activity; protein binding Biological Process: positive regulation of receptor-mediated endocytosis; regulation of exocytosis |
NCBI Summary: | This gene belongs to the Rab family of monomeric GTPases, which are involved in the control of cellular membrane traffic. The encoded protein plays a role in the targeted trafficking of integrins via its association with integrin alpha tails. As a consequence, the encoded protein is involved in the regulation of cell adhesion and migration. Expression of this gene is associated with a poor prognosis for glioma patients. This gene is downregulated by the tumor suppressor miR-200b, and miRNA-200b is itself downregulated in glioma tissues. [provided by RefSeq, Nov 2015] |
UniProt Code: | Q9UL25 |
NCBI GenInfo Identifier: | 13633613 |
NCBI Gene ID: | 23011 |
NCBI Accession: | Q9UL25.3 |
UniProt Secondary Accession: | Q9UL25,Q14466, Q569H3, |
UniProt Related Accession: | Q9UL25 |
Molecular Weight: | 24,348 Da |
NCBI Full Name: | Ras-related protein Rab-21 |
NCBI Synonym Full Names: | RAB21, member RAS oncogene family |
NCBI Official Symbol: | RAB21 |
NCBI Protein Information: | ras-related protein Rab-21 |
UniProt Protein Name: | Ras-related protein Rab-21 |
Protein Family: | Ras-related protein |
UniProt Gene Name: | RAB21 |
UniProt Entry Name: | RAB21_HUMAN |
Antibodies |
Anti-RAB21 Antibody (CAB12095) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |