PUS10 Antibody (PACO20297)
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주문- SKU:
- PACO20297
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | PUS10 Antibody (PACO20297) |
Antibody SKU: | PACO20297 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:50-1:200 |
Species Reactivity: | Human, Mouse |
Immunogen: | Synthetic peptide of human PUS10 |
Form: | Liquid |
Storage Buffer: | -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
Purification Method: | Antigen affinity purification |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using PACO20297(PUS10 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200). | |
Gel: 8%SDS-PAGE, Lysate: 40 µg, Lane: Mouse intestinum tenue tissue, Primary antibody: PACO20297(PUS10 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 seconds. | |
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using PACO20297(PUS10 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200). |
Background: | Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs. |
Synonyms: | pseudouridylate synthase 10 |
UniProt Protein Function: | PUS10: Pseudouridylate synthases catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. PUS10 catalyzes the formation of the universal psi55 in the GC loop of transfer RNAs (Probable). Modulator of TRAIL-induced cell death via activation of procaspase 8 and BID cleavage. Required for the progression of the apoptotic signal through intrinsic mitochondrial cell death. Belongs to the pseudouridine synthase Pus10 family. |
UniProt Protein Details: | Protein type:RNA processing; Isomerase; EC 5.4.99.- Chromosomal Location of Human Ortholog: 2p16.1 Molecular Function:RNA binding; pseudouridine synthase activity Biological Process: pseudouridine synthesis; tRNA processing |
NCBI Summary: | Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty et al., 2007 [PubMed 17900615]).[supplied by OMIM, May 2009] |
UniProt Code: | Q3MIT2 |
NCBI GenInfo Identifier: | 207113130 |
NCBI Gene ID: | 150962 |
NCBI Accession: | NP_653310.2 |
UniProt Secondary Accession: | Q3MIT2,Q5JPJ5, Q96MI8, |
UniProt Related Accession: | Q3MIT2 |
Molecular Weight: | 60,244 Da |
NCBI Full Name: | putative tRNA pseudouridine synthase Pus10 |
NCBI Synonym Full Names: | pseudouridylate synthase 10 |
NCBI Official Symbol: | PUS10 |
NCBI Official Synonym Symbols: | DOBI; CCDC139 |
NCBI Protein Information: | putative tRNA pseudouridine synthase Pus10 |
UniProt Protein Name: | Putative tRNA pseudouridine synthase Pus10 |
UniProt Synonym Protein Names: | Coiled-coil domain-containing protein 139; tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase |
Protein Family: | Putative tRNA pseudouridine synthase |
UniProt Gene Name: | PUS10 |
UniProt Entry Name: | PUS10_HUMAN |
Antibodies |
Anti-PUS10 Antibody (CAB15962) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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