PTRH2 Antibody (PACO16916)
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주문- SKU:
- PACO16916
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | PTRH2 Antibody (PACO16916) |
Antibody SKU: | PACO16916 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:10-1:50 |
Species Reactivity: | Human, Mouse |
Immunogen: | Fusion protein of human PTRH2 |
Form: | Liquid |
Storage Buffer: | -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
Purification Method: | Antigen affinity purification |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using PACO16916(PTRH2 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200). | |
Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-5: K562 cells, hepg2 cells, 231 cells, Jurkat cells, Raji cells, Primary antibody: PACO16916(PTRH2 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds. | |
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using PACO16916(PTRH2 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200). |
Background: | PTRH2 (peptidyl-tRNA hydrolase 2), also known as BIT1 (Bcl-2 inhibitor of transcription 1), is a 179 amino acid, mitochondrial protein. During apoptosis, PTRH2 is released from the mitochondia to the cytoplasm. Once in the cytoplasm, PTRH2 regulates the function of two transcriptional regulators, TLE5 and TLE1, thereby promoting caspase-independent cell death. It is also believed that the natural substrate for PTRH2 may be petidyl-tRNAs, which leave the ribosomes during protein synthesis. |
Synonyms: | peptidyl-tRNA hydrolase 2 |
UniProt Protein Function: | PTRH2: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis. Monomer. Belongs to the PTH2 family. |
UniProt Protein Details: | Protein type:EC 3.1.1.29; Mitochondrial; Hydrolase Chromosomal Location of Human Ortholog: 17q23.1 Cellular Component: membrane; mitochondrion; cytosol Molecular Function:protein binding; aminoacyl-tRNA hydrolase activity Biological Process: apoptosis; metabolic process Disease: Neurologic, Endocrine, And Pancreatic Disease, Multisystem, Infantile-onset |
NCBI Summary: | The protein encoded by this gene is a mitochondrial protein with two putative domains, an N-terminal mitochondrial localization sequence, and a UPF0099 domain. In vitro assays suggest that this protein possesses peptidyl-tRNA hydrolase activity, to release the peptidyl moiety from tRNA, thereby preventing the accumulation of dissociated peptidyl-tRNA that could reduce the efficiency of translation. This protein also plays a role regulating cell survival and death. It promotes survival as part of an integrin-signaling pathway for cells attached to the extracellular matrix (ECM), but also promotes apoptosis in cells that have lost their attachment to the ECM, a process called anoikos. After loss of cell attachment to the ECM, this protein is phosphorylated, is released from the mitochondria into the cytosol, and promotes caspase-independent apoptosis through interactions with transcriptional regulators. This gene has been implicated in the development and progression of tumors, and mutations in this gene have been associated with an infantile multisystem neurologic, endocrine, and pancreatic disease (INMEPD) characterized by intellectual disability, postnatal microcephaly, progressive cerebellar atrophy, hearing impairment, polyneuropathy, failure to thrive, and organ fibrosis with exocrine pancreas insufficiency (PMID: 25574476). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015] |
UniProt Code: | Q9Y3E5 |
NCBI GenInfo Identifier: | 6686183 |
NCBI Gene ID: | 51651 |
NCBI Accession: | Q9Y3E5.1 |
UniProt Secondary Accession: | Q9Y3E5,Q9NTE5, B3KUY4, |
UniProt Related Accession: | Q9Y3E5 |
Molecular Weight: | 179 |
NCBI Full Name: | Peptidyl-tRNA hydrolase 2, mitochondrial |
NCBI Synonym Full Names: | peptidyl-tRNA hydrolase 2 |
NCBI Official Symbol: | PTRH2 |
NCBI Official Synonym Symbols: | BIT1; PTH2; CFAP37; CGI-147 |
NCBI Protein Information: | peptidyl-tRNA hydrolase 2, mitochondrial; PTH 2; bcl-2 inhibitor of transcription 1; cilia and flagella associated protein 37 |
UniProt Protein Name: | Peptidyl-tRNA hydrolase 2, mitochondrial |
UniProt Synonym Protein Names: | Bcl-2 inhibitor of transcription 1 |
UniProt Gene Name: | PTRH2 |
UniProt Entry Name: | PTH2_HUMAN |