PSME4 Antibody (PACO60012)
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주문- SKU:
- PACO60012
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | PSME4 Antibody (PACO60012) |
Antibody SKU: | PACO60012 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Proteasome activator complex subunit 4 protein (105-198AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: K562 whole cell lysate. All lanes: PSME4 antibody at 4.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 212, 140, 114, 25 kDa. Observed band size: 212 kDa. | |
IHC image of PACO60012 diluted at 1:600 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
IHC image of PACO60012 diluted at 1:600 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | Associated component of the proteasome that specifically recognizes acetylated histones and promotes ATP- and ubiquitin-independent degradation of core histones during spermatogenesis and DNA damage response. Recognizes and binds acetylated histones via its bromodomain-like (BRDL) region and activates the proteasome by opening the gated channel for substrate entry. Binds to the core proteasome via its C-terminus, which occupies the same binding sites as the proteasomal ATPases, opening the closed structure of the proteasome via an active gating mechanism. Component of the spermatoproteasome, a form of the proteasome specifically found in testis: binds to acetylated histones and promotes degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis. Also involved in DNA damage response in somatic cells, by promoting degradation of histones following DNA double-strand breaks. |
Synonyms: | Proteasome activator complex subunit 4 (Proteasome activator PA200), PSME4, KIAA0077 |
UniProt Protein Function: | Associated component of the proteasome that specifically recognizes acetylated histones and promotes ATP- and ubiquitin-independent degradation of core histones during spermatogenesis and DNA damage response. Recognizes and binds acetylated histones via its bromodomain-like (BRDL) region and activates the proteasome by opening the gated channel for substrate entry. Binds to the core proteasome via its C-terminus, which occupies the same binding sites as the proteasomal ATPases, opening the closed structure of the proteasome via an active gating mechanism. Component of the spermatoproteasome, a form of the proteasome specifically found in testis: binds to acetylated histones and promotes degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis. Also involved in DNA damage response in somatic cells, by promoting degradation of histones following DNA double-strand breaks. |
UniProt Code: | Q14997 |
NCBI GenInfo Identifier: | 163644283 |
NCBI Gene ID: | 23198 |
NCBI Accession: | NP_055429.2 |
UniProt Secondary Accession: | Q14997,Q1XBG4, Q1XBG5, Q1XBG6, Q2M1Z0, Q6IPR2, Q86XF8 |
UniProt Related Accession: | Q14997 |
Molecular Weight: | Calculated: 24kDa; 113kDa; 139kDa; 211kDaObserved: 211kDa |
NCBI Full Name: | proteasome activator complex subunit 4 |
NCBI Synonym Full Names: | proteasome activator subunit 4 |
NCBI Official Symbol: | PSME4 |
NCBI Official Synonym Symbols: | PA200 |
NCBI Protein Information: | proteasome activator complex subunit 4 |
UniProt Protein Name: | Proteasome activator complex subunit 4 |
UniProt Synonym Protein Names: | Proteasome activator PA200 |
Protein Family: | Proteasome activator complex |
UniProt Gene Name: | PSME4 |
Antibodies |
Anti-PSME4 Antibody (CAB13815) |
Anti-PSME4 Antibody (CAB19873)[KO Validated] |
PSME4 Antibody (PACO11559) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |