PRKRA Antibody (PACO33364)
제품 코드를 사용하여 Assay Genie 메인 사이트를 통해 주문합니다.
주문- SKU:
- PACO33364
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | PRKRA Antibody (PACO33364) |
Antibody SKU: | PACO33364 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:500 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Interferon-inducible double-stranded RNA-dependent protein kinase activator A protein (1-313AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Immunohistochemistry of paraffin-embedded human prostate cancer using PACO33364 at dilution of 1:100. | |
Immunofluorescence staining of HepG2 cells with PACO33364 at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Background: | Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-α and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner (By similarity). |
Synonyms: | Interferon-inducible double-stranded RNA-dependent protein kinase activator A (PKR-associated protein X) (PKR-associating protein X) (Protein activator of the interferon-induced protein kinase) (Protein kinase, interferon-inducible double-stranded RNA-dependent activator), PRKRA, PACT RAX |
UniProt Protein Function: | PACT: a double strand RNA binding protein (dsRBP). Interacts with TRBP and Dicer to stimulate the cleavage of double-stranded or short hairpin RNA to siRNA. Appears to have a pro-apoptotic function that may be suppressed in the presence of growth factor. Activates the interferon-induced protein kinase PKR. Three alternatively spliced human isoforms have been reported. |
UniProt Protein Details: | Protein type:Activator; RNA-binding; RNA processing Chromosomal Location of Human Ortholog: 2q31.2 Cellular Component: nucleoplasm; membrane; perinuclear region of cytoplasm; cytoplasm; cytosol Molecular Function:protein binding; protein homodimerization activity; enzyme activator activity Biological Process: positive regulation of catalytic activity; negative regulation of cell proliferation; skeletal morphogenesis; response to virus; outer ear morphogenesis; immune response; gene expression; middle ear morphogenesis; RNA interference, production of siRNA; protein amino acid phosphorylation Disease: Dystonia 16 |
NCBI Summary: | This gene encodes a protein kinase activated by double-stranded RNA which mediates the effects of interferon in response to viral infection. Mutations in this gene have been associated with dystonia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008] |
UniProt Code: | O75569 |
NCBI GenInfo Identifier: | 74735517 |
NCBI Gene ID: | 8575 |
NCBI Accession: | O75569.1 |
UniProt Secondary Accession: | O75569,Q53G24, Q6X7T5, Q8NDK4, A8K3I6, |
UniProt Related Accession: | O75569 |
Molecular Weight: | 313 |
NCBI Full Name: | Interferon-inducible double-stranded RNA-dependent protein kinase activator A |
NCBI Synonym Full Names: | protein kinase, interferon-inducible double stranded RNA dependent activator |
NCBI Official Symbol: | PRKRA |
NCBI Official Synonym Symbols: | RAX; PACT; DYT16; HSD14 |
NCBI Protein Information: | interferon-inducible double-stranded RNA-dependent protein kinase activator A; PKR-associated protein X; PKR-associating protein X; protein activator of the interferon-induced protein kinase; protein kinase, interferon-inducible double stranded RNA-dependent activator; protein kinase, interferon-inducible double-stranded RNA-dependent activator; interferon-inducible double stranded RNA-dependent protein kinase activator A |
UniProt Protein Name: | Interferon-inducible double-stranded RNA-dependent protein kinase activator A |
UniProt Synonym Protein Names: | PKR-associated protein X; PKR-associating protein X; Protein activator of the interferon-induced protein kinase; Protein kinase, interferon-inducible double-stranded RNA-dependent activator |
Protein Family: | Interferon-inducible double-stranded RNA-dependent protein kinase activator |
UniProt Gene Name: | PRKRA |
UniProt Entry Name: | PRKRA_HUMAN |