PIAS4 Antibody (PACO23709)
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주문- SKU:
- PACO23709
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | PIAS4 Antibody (PACO23709) |
Antibody SKU: | PACO23709 |
Size: | 100ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:3000, IHC:1:50-1:100 |
Species Reactivity: | Human, Mouse |
Immunogen: | Synthesized peptide derived from Internal of human PIAS4. |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using PIAS4 antibody. | |
Western blot analysis of extracts from JK cells (Lane 2), using PIAS4 antiobdy. The lane on the left is treated with synthesized peptide. | |
Western blot analysis of extracts from Jurkat cells, using PIAS4 antibody. |
Background: | Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. |
Synonyms: | PIAS-gamma; PIASG; PIASy; protein inhibitor of activated STAT protein 4; protein inhibitor of activated STAT protein PIASy |
UniProt Protein Function: | PIAS4: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Promotes PARK7 sumoylation. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Interacts with AR, AXIN1, GATA2, LEF1, TP53 and STAT1 (IFNG-induced). Binds to AT-rich DNA sequences, known as matrix or scaffold attachment regions (MARs/SARs). Interacts with TICAM1. Interacts with KLF8; the interaction results in SUMO ligation and repression of KLF8 transcriptional activity and of its cell cycle progression into G(1) phase. Highly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes. Belongs to the PIAS family. |
UniProt Protein Details: | Protein type:SUMO conjugating system; Transcription, coactivator/corepressor; EC 6.3.2.-; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: cytoplasm; nucleoplasm; nucleus Molecular Function:protein binding; SUMO ligase activity; ubiquitin protein ligase binding Biological Process: double-strand break repair via nonhomologous end joining; negative regulation of transcription, DNA-dependent; positive regulation of protein sumoylation; protein sumoylation |
UniProt Code: | Q8N2W9 |
NCBI GenInfo Identifier: | 34922831 |
NCBI Gene ID: | 51588 |
NCBI Accession: | Q8N2W9.1 |
UniProt Secondary Accession: | Q8N2W9,O75926, Q96G19, Q9UN16, |
UniProt Related Accession: | Q8N2W9 |
Molecular Weight: | 56,504 Da |
NCBI Full Name: | E3 SUMO-protein ligase PIAS4 |
NCBI Synonym Full Names: | protein inhibitor of activated STAT 4 |
NCBI Official Symbol: | PIAS4 |
NCBI Official Synonym Symbols: | PIASY; Piasg; ZMIZ6; PIAS-gamma |
NCBI Protein Information: | E3 SUMO-protein ligase PIAS4 |
UniProt Protein Name: | E3 SUMO-protein ligase PIAS4 |
UniProt Synonym Protein Names: | PIASy; Protein inhibitor of activated STAT protein 4; Protein inhibitor of activated STAT protein gamma; PIAS-gamma |
Protein Family: | E3 SUMO-protein ligase |
UniProt Gene Name: | PIAS4 |
UniProt Entry Name: | PIAS4_HUMAN |
Antibodies | ELISA Kits |
Anti-PIAS4 Antibody (CAB5322) | PIAS4 Colorimetric Cell-Based ELISA Kit |
PIAS4 Antibody (PACO04550) | |
PIAS4 Antibody (PACO11233) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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