PIAS2 Antibody (PACO23145)
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주문- SKU:
- PACO23145
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | PIAS2 Antibody (PACO23145) |
Antibody SKU: | PACO23145 |
Size: | 100ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:3000 |
Species Reactivity: | Human, Mouse, Rat |
Immunogen: | Synthesized peptide derived from Internal of human PIAS2. |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot analysis of extracts from COS7 cells, using PIAS2 antibody. | |
Western blot analysis of extracts from HuvEc cells (Lane 2), JK cells (Lane 3) and cos-7 cells (Lane 4), using PIAS2 antiobdy. The lane on the left is treated with synthesized peptide. |
Background: | Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha. Isoform PIAS2-alpha sumoylates PML at'Lys-65' and 'Lys-160'. |
Synonyms: | ARIP3; Androgen receptor-interacting protein 3; DAB2-interacting protein; DIP; Miz1 |
UniProt Protein Function: | Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha. Isoform PIAS2-alpha sumoylates PML at'Lys-65' and 'Lys-160'. |
NCBI Summary: | This gene encodes a member of the protein inhibitor of activated STAT (PIAS) family. PIAS proteins function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Isoforms of the encoded protein enhance the sumoylation of specific target proteins including the p53 tumor suppressor protein, c-Jun, and the androgen receptor. A pseudogene of this gene is located on the short arm of chromosome 4. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1. [provided by RefSeq, Aug 2011] |
UniProt Code: | O75928 |
NCBI GenInfo Identifier: | 229463031 |
NCBI Gene ID: | 9063 |
NCBI Accession: | O75928.3 |
UniProt Secondary Accession: | O75928,O75927, Q96BT5, Q96KE3, |
UniProt Related Accession: | O75928 |
Molecular Weight: | 45,057 Da |
NCBI Full Name: | E3 SUMO-protein ligase PIAS2 |
NCBI Synonym Full Names: | protein inhibitor of activated STAT 2 |
NCBI Official Symbol: | PIAS2 |
NCBI Official Synonym Symbols: | DIP; MIZ; MIZ1; SIZ2; ARIP3; PIASX; ZMIZ4; PIASX-BETA; PIASX-ALPHA |
NCBI Protein Information: | E3 SUMO-protein ligase PIAS2 |
UniProt Protein Name: | E3 SUMO-protein ligase PIAS2 |
UniProt Synonym Protein Names: | Androgen receptor-interacting protein 3; ARIP3; DAB2-interacting protein; DIP; Msx-interacting zinc finger protein; Miz1; PIAS-NY protein; Protein inhibitor of activated STAT x; Protein inhibitor of activated STAT2 |
Protein Family: | E3 SUMO-protein ligase |
UniProt Gene Name: | PIAS2 |
UniProt Entry Name: | PIAS2_HUMAN |
Antibodies |
Anti-PIAS2 Antibody (CAB5654) |
Anti-PIAS2 Antibody (CAB5203) |
PIAS2 Antibody (PACO06751) |
PIAS2 Antibody (PACO11231) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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