Phospho-UBTF (Ser484) Antibody (PACO24435)
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주문- SKU:
- PACO24435
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | Phospho-UBTF (Ser484) Antibody (PACO24435) |
Antibody SKU: | PACO24435 |
Size: | 100ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:3000, IHC:1:50-1:100 |
Species Reactivity: | Human, Mouse, Rat |
Immunogen: | Peptide sequence around phosphorylation site of serine 484 (P-E-S(p)-P-K) derived from Human UBF. |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot analysis of extracts from HeLa cells, treated with calyculinA (50ng/ml, 30mins), using UBF (Phospho-Ser484) antibody. The lane on the right is treated with the synthesized peptide. | |
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using UBF (Phospho-Ser484) antibody. The picture on the right is treated with the synthesized peptide. |
Background: | Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element. |
Synonyms: | Autoantigen NOR-90; Nucleolar transcription factor 1; TCFUBF; UBF-1; UBF1; UBTF; Upstream binding factor 1 |
UniProt Protein Function: | Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element. |
NCBI Summary: | This gene encodes a member of the HMG-box DNA-binding protein family. The encoded protein plays a critical role in ribosomal RNA transcription as a key component of the pre-initiation complex, mediating the recruitment of RNA polymerase I to rDNA promoter regions. The encoded protein may also play important roles in chromatin remodeling and pre-rRNA processing, and its activity is regulated by both phosphorylation and acetylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3, 11 and X and the long arm of chromosome 11. [provided by RefSeq, Aug 2011] |
UniProt Code: | P17480 |
NCBI GenInfo Identifier: | 136652 |
NCBI Gene ID: | 7343 |
NCBI Accession: | P17480.1 |
UniProt Secondary Accession: | P17480,A8K6R8, |
UniProt Related Accession: | P17480 |
Molecular Weight: | 84,937 Da |
NCBI Full Name: | Nucleolar transcription factor 1 |
NCBI Synonym Full Names: | upstream binding transcription factor, RNA polymerase I |
NCBI Official Symbol: | UBTF |
NCBI Official Synonym Symbols: | UBF; UBF1; UBF2; UBF-1; NOR-90 |
NCBI Protein Information: | nucleolar transcription factor 1 |
UniProt Protein Name: | Nucleolar transcription factor 1 |
UniProt Synonym Protein Names: | Autoantigen NOR-90; Upstream-binding factor 1; UBF-1 |
Protein Family: | Nucleolar transcription factor |
UniProt Gene Name: | UBTF |
UniProt Entry Name: | UBF1_HUMAN |
Antibodies | ELISA Kits |
Anti-UBTF Antibody (CAB9847) | UBF1 Colorimetric Cell-Based ELISA Kit |
Phospho-UBTF (S484) Antibody (PACO05276) | |
UBTF Antibody (PACO05277) | |
UBTF Antibody (PACO06848) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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