Phospho-RPS6KA5 (Ser212) Antibody (PACO24025)
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주문- SKU:
- PACO24025
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | Phospho-RPS6KA5 (Ser212) Antibody (PACO24025) |
Antibody SKU: | PACO24025 |
Size: | 100ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:1000 |
Species Reactivity: | Human, Mouse |
Immunogen: | Peptide sequence around phosphorylation site of serine 212 (A-Y-S(p)-F-C) derived from Human MSK1. |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot analysis of extracts from NIH-3T3 cells treated with EGF using MSK1 (Phospho-Ser212) antibody.The lane on the right is treated with the antigen-specific peptide. |
Background: | Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14). |
Synonyms: | polypeptide 5; KS6A5; MSPK1; RPS6KA5; RLPK |
UniProt Protein Function: | MSK1: an AGC kinase of the RSK family. Has two kinase domains connected by a regulatory linker region. Enzyme activity requires the presence of both kinase domains. Is phosphorylated and activated by Erk as well as p38 MAPK in response to growth factors and cellular stress. |
UniProt Protein Details: | Protein type:EC 2.7.11.1; Protein kinase, AGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); AGC group; RSK family; MSK subfamily Chromosomal Location of Human Ortholog: 14q31-q32.1 Cellular Component: cytoplasm; nucleoplasm; nucleus Molecular Function:ATP binding; histone serine kinase activity (H3-S10 specific); magnesium ion binding; protein binding; protein kinase activity; protein serine/threonine kinase activity Biological Process: activation of CREB transcription factor; activation of NF-kappaB transcription factor; axon guidance; epidermal growth factor receptor signaling pathway; histone phosphorylation; inflammatory response; innate immune response; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of cytokine production; negative regulation of transcription, DNA-dependent; nerve growth factor receptor signaling pathway; positive regulation of histone acetylation; positive regulation of histone phosphorylation; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; regulation of transcription, DNA-dependent; stimulatory C-type lectin receptor signaling pathway; stress-activated MAPK cascade; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway |
UniProt Code: | O75582 |
NCBI GenInfo Identifier: | 37999482 |
NCBI Gene ID: | 9252 |
NCBI Accession: | O75582.1 |
UniProt Secondary Accession: | O75582,O95316, Q96AF7, B7Z2Y5, |
UniProt Related Accession: | O75582 |
Molecular Weight: | 81,780 Da |
NCBI Full Name: | Ribosomal protein S6 kinase alpha-5 |
NCBI Synonym Full Names: | ribosomal protein S6 kinase A5 |
NCBI Official Symbol: | RPS6KA5 |
NCBI Official Synonym Symbols: | MSK1; RLPK; MSPK1 |
NCBI Protein Information: | ribosomal protein S6 kinase alpha-5 |
UniProt Protein Name: | Ribosomal protein S6 kinase alpha-5 |
UniProt Synonym Protein Names: | 90 kDa ribosomal protein S6 kinase 5; Nuclear mitogen- and stress-activated protein kinase 1; RSK-like protein kinase; RSKL |
Protein Family: | Ribosomal protein S6 kinase |
UniProt Gene Name: | RPS6KA5 |
UniProt Entry Name: | KS6A5_HUMAN |
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