Phospho-RASGRF1 (Ser916) Antibody (PACO24090)
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주문- SKU:
- PACO24090
- Product type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | Phospho-RASGRF1 (Ser916) Antibody (PACO24090) |
Antibody SKU: | PACO24090 |
Size: | 100ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:50-1:100 |
Species Reactivity: | Mouse, Rat |
Immunogen: | Peptide sequence around phosphorylation site of Serine 916(N-E-G(p)-T P) derived from Human Ras-GRF1. |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Immunohistochemical analysis of paraffin-embedded human brain tissue using Ras-GRF1 (Phospho-Ser916) antibody (left)or the same antibody preincubated with blocking peptide (right). |
Background: | Ras activity is regulated by GAP (GTPase activating proteins) and GEFs (guanine nucleotide exchange factors). Ras-GRF1 (also known as CDC25Mm) is neuronal RasGEF and is regulated by heterotrimeric G proteins and calcium influx . Binding to calmodulin and phosphorylation stimulate Ras-GRF1 activity. Multiple PKA phosphorylation sites on Ras-GRF have been identified. Phosphorylation on the two major sites, Ser54 and Ser822, inhibits Ras-GRF activity. Carbachol (a muscarinic agonist)-induced phosphorylation on Ser916 is essential but not sufficient for maximal Ras-GRF activity. |
Synonyms: | CDC25; GNRP; GRF1; RASGRF1; |
UniProt Protein Function: | RASGRF1: a guanine nucleotide exchange factor (GEF). Stimulates the dissociation of GDP from RAS protein. Activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit in the mouse brain,. The signaling pathway mediated by this protein may be important for long-term memory. Two alternatively spliced variants have been reported. |
UniProt Protein Details: | Protein type:GEFs, Ras; GEFs Chromosomal Location of Human Ortholog: 15q24.2 Cellular Component: cytosol; growth cone; plasma membrane Molecular Function:guanyl-nucleotide exchange factor activity; Ras guanyl-nucleotide exchange factor activity Biological Process: MAPKKK cascade; neurite development; positive regulation of GTPase activity; positive regulation of Ras protein signal transduction; regulation of Rac protein signal transduction; regulation of Ras protein signal transduction; regulation of synaptic plasticity; signal transduction |
NCBI Summary: | The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Mar 2009] |
UniProt Code: | Q13972 |
NCBI GenInfo Identifier: | 613504205 |
NCBI Gene ID: | 5923 |
NCBI Accession: | Q13972.2 |
UniProt Secondary Accession: | Q13972,Q16027, F8VPA5, H0YKF2, J3KQP9, |
UniProt Related Accession: | Q13972 |
Molecular Weight: | 143,218 Da |
NCBI Full Name: | Ras-specific guanine nucleotide-releasing factor 1 |
NCBI Synonym Full Names: | Ras protein specific guanine nucleotide releasing factor 1 |
NCBI Official Symbol: | RASGRF1 |
NCBI Official Synonym Symbols: | GNRP; GRF1; CDC25; GRF55; CDC25L; H-GRF55; PP13187; ras-GRF1 |
NCBI Protein Information: | ras-specific guanine nucleotide-releasing factor 1 |
UniProt Protein Name: | Ras-specific guanine nucleotide-releasing factor 1 |
UniProt Synonym Protein Names: | Guanine nucleotide-releasing protein; GNRP; Ras-specific nucleotide exchange factor CDC25 |
Protein Family: | Ras-specific guanine nucleotide-releasing factor |
UniProt Gene Name: | RASGRF1 |
UniProt Entry Name: | RGRF1_HUMAN |