Phospho-PLD1 (Thr147) Antibody (PACO24162)
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주문- SKU:
- PACO24162
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | Phospho-PLD1 (Thr147) Antibody (PACO24162) |
Antibody SKU: | PACO24162 |
Size: | 100ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:1000 |
Species Reactivity: | Human, Mouse, Rat |
Immunogen: | Peptide sequence around phosphorylation site of threonine 147(R-H-T(p)-F-R) derived from Human PLD1. |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot analysis of extracts from HepG2 cells using PLD1 (Phospho-Thr147) Antibody.The lane on the right is treated with the antigen-specific peptide. |
Background: | Phosphatidylcholine (PC)-specific phospholipases D (PLDs; EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid, and choline. A range of agonists acting through G protein-coupled receptors and receptor tyrosine kinases stimulate this hydrolysis. PC-specific PLD activity has been implicated in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. |
Synonyms: | PLD 1; hPLD1; Choline phosphatase 1; |
UniProt Protein Function: | PLD1: a phosphatidylcholine-hydrolyzing phospholipase. Activated by ADP-ribosylated Rho and protein kinase C. Implicated as a critical step in numerous cellular pathways including membrane trafficking and the regulation polymorphonuclear leukocyte degranulation and oxidant production. May be involved in the regulation of perinuclear intravesicular membrane traffic. Four splice-variant isoforms have been described. |
UniProt Protein Details: | Protein type:Phospholipase; Lipid Metabolism - glycerophospholipid; Motility/polarity/chemotaxis; EC 3.1.4.4; Lipid Metabolism - ether lipid Chromosomal Location of Human Ortholog: 3q26 Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; membrane; perinuclear region of cytoplasm; late endosome membrane; lysosomal membrane; endocytic vesicle; apical plasma membrane; endosome Molecular Function:protein binding; phospholipase D activity; phosphoinositide binding Biological Process: defense response to Gram-positive bacterium; phosphatidylglycerol biosynthetic process; small GTPase mediated signal transduction; phospholipid metabolic process; glycerophospholipid biosynthetic process; Ras protein signal transduction; phosphatidic acid biosynthetic process; chemotaxis; lipid catabolic process; regulation of microvillus biogenesis |
NCBI Summary: | This gene encodes a phosphatidylcholine-specific phospholipase which catalyzes the hydrolysis of phosphatidylcholine in order to yield phosphatidic acid and choline. The enzyme may play a role in signal transduction and subcellular trafficking. Alternative splicing results in multiple transcript variants with both catalytic and regulatory properties. [provided by RefSeq, Sep 2011] |
UniProt Code: | Q13393 |
NCBI GenInfo Identifier: | 4505873 |
NCBI Gene ID: | 5337 |
NCBI Accession: | NP_002653.1 |
UniProt Related Accession: | Q13393 |
Molecular Weight: | |
NCBI Full Name: | phospholipase D1 isoform a |
NCBI Synonym Full Names: | phospholipase D1 |
NCBI Official Symbol: | PLD1 |
NCBI Official Synonym Symbols: | CVDD |
NCBI Protein Information: | phospholipase D1 |
UniProt Protein Name: | Phospholipase D1 |
UniProt Synonym Protein Names: | Choline phosphatase 1; Phosphatidylcholine-hydrolyzing phospholipase D1 |
Protein Family: | Phospholipase |
UniProt Gene Name: | PLD1 |
UniProt Entry Name: | PLD1_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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