Phospho-OPRM1 (Ser375) Antibody (PACO23924)
제품 코드를 사용하여 Assay Genie 메인 사이트를 통해 주문합니다.
주문- SKU:
- PACO23924
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | Phospho-OPRM1 (Ser375) Antibody (PACO23924) |
Antibody SKU: | PACO23924 |
Size: | 100ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:1000 |
Species Reactivity: | Human, Mouse, Rat |
Immunogen: | Peptide sequence around phosphorylation site of serine 375(H-P-S(p)-T-A) derived from Human Opioid Receptor. |
Form: | Liquid |
Storage Buffer: | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot analysis of extracts from Rat brain tissue and Mouse brain tissue using Opioid Receptor (Phospho-Ser375) Antibody. |
Background: | Receptor for endogenous opioids such as beta-endorphin and endomorphin. Receptor for natural and synthetic opioids including morphine, heroin, DAMGO, fentanyl, etorphine, buprenorphin and methadone. Agonist binding to the receptor induces coupling to an inactive GDP-bound heterotrimeric G-protein complex and subsequent exchange of GDP for GTP in the G-protein alpha subunit leading to dissociation of the G-protein complex with the free GTP-bound G-protein alpha and the G-protein beta-gamma dimer activating downstream cellular effectors. The agonist- and cell type-specific activity is predominantly coupled to pertussis toxin-sensitive G(i) and G(o) G alpha proteins, GNAI1, GNAI2, GNAI3 and GNAO1 isoforms Alpha-1 and Alpha-2, and to a lesser extend to pertussis toxin-insensitive G alpha proteins GNAZ and GNA15. They mediate an array of downstream cellular responses, including inhibition of adenylate cyclase activity and both N-type and L-type calcium channels, activation of inward rectifying potassium channels, mitogen-activated protein kinase (MAPK), phospholipase C (PLC), phosphoinositide/protein kinase (PKC), phosphoinositide 3-kinase (PI3K) and regulation of NF-kappa-B. Also couples to adenylate cyclase stimulatory G alpha proteins. The selective temporal coupling to G-proteins and subsequent signaling can be regulated by RGSZ proteins, such as RGS9, RGS17 and RGS4. Phosphorylation by members of the GPRK subfamily of Ser/Thr protein kinases and association with beta-arrestins is involved in short-term receptor desensitization. Beta-arrestins associate with the GPRK-phosphorylated receptor and uncouple it from the G-protein thus terminating signal transduction. The phosphorylated receptor is internalized through endocytosis via clathrin-coated pits which involves beta-arrestins. The activation of the ERK pathway occurs either in a G-protein-dependent or a beta-arrestin-dependent manner and is regulated by agonist-specific receptor phosphorylation. Acts as a class A G-protein coupled receptor (GPCR) which dissociates from beta-arrestin at or near the plasma membrane and undergoes rapid recycling. Receptor down-regulation pathways are varying with the agonist and occur dependent or independent of G-protein coupling. Endogenous ligands induce rapid desensitization, endocytosis and recycling whereas morphine induces only low desensitization and endocytosis. Heterooligomerization with other GPCRs can modulate agonist binding, signaling and trafficking properties. Involved in neurogenesis. Isoform 12 couples to GNAS and is proposed to be involved in excitatory effects. Isoform 16 and isoform 17 do not bind agonists but may act through oligomerization with binding-competent OPRM1 isoforms and reduce their ligand binding activity. |
Synonyms: | MOP; MOR; LMOR; MOR1; OPRM |
UniProt Protein Function: | MOR-1: a Gi-protein-coupled receptor for beta-endorphin, morphine and other opiates. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Ligand-binding inactivates adenylyl cyclase, and activates a variety of G-beta-gamma-dependent pathways including the MAPK and the PI3K/Akt cascades. Two splice-variant isoforms have been described. |
UniProt Protein Details: | Protein type:GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral Chromosomal Location of Human Ortholog: 6q24-q25 Cellular Component: dendrite cytoplasm; Golgi apparatus; neuron projection; focal adhesion; endoplasmic reticulum; integral to plasma membrane; plasma membrane; perikaryon; sarcolemma; lipid raft Molecular Function:G-protein coupled receptor activity; voltage-gated calcium channel activity; neuropeptide binding; protein domain specific binding; protein binding; G-protein alpha-subunit binding; G-protein beta-subunit binding; filamin binding; beta-endorphin receptor activity Biological Process: response to food; positive regulation of neurogenesis; positive regulation of nitric oxide biosynthetic process; wound healing; negative regulation of nitric oxide biosynthetic process; cellular response to stress; negative regulation of adenylate cyclase activity; locomotory behavior; response to lipopolysaccharide; behavioral response to ethanol; sensory perception of pain; response to cocaine; G-protein signaling, coupled to cyclic nucleotide second messenger; negative regulation of cell proliferation; synaptic transmission; elevation of cytosolic calcium ion concentration; neuropeptide signaling pathway; reduction of cytosolic calcium ion concentration; positive regulation of appetite; sensory perception; opioid receptor, adenylate cyclase inhibiting pathway; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); regulation of sensory perception of pain; regulation of excitatory postsynaptic membrane potential; dopamine receptor, adenylate cyclase activating pathway; acute inflammatory response to antigenic stimulus Disease: Epilepsy, Idiopathic Generalized |
NCBI Summary: | This gene encodes one of at least three opioid receptors in humans; the mu opioid receptor (MOR). The MOR is the principal target of endogenous opioid peptides and opioid analgesic agents such as beta-endorphin and enkephalins. The MOR also has an important role in dependence to other drugs of abuse, such as nicotine, cocaine, and alcohol via its modulation of the dopamine system. The NM_001008503.2:c.118A>G allele has been associated with opioid and alcohol addiction and variations in pain sensitivity but evidence for it having a causal role is conflicting. Multiple transcript variants encoding different isoforms have been found for this gene. Though the canonical MOR belongs to the superfamily of 7-transmembrane-spanning G-protein-coupled receptors some isoforms of this gene have only 6 transmembrane domains. [provided by RefSeq, Oct 2013] |
UniProt Code: | P35372 |
NCBI GenInfo Identifier: | 2851402 |
NCBI Gene ID: | 4988 |
NCBI Accession: | P35372.2 |
UniProt Secondary Accession: | P35372,Q12930, Q4VWM1, Q4VWM2, B0FXJ1, B2R9S7, B8Q1L7 B8Q1L8, B8Q1L9, E7EWZ3, G8XRH6, G8XRH8, |
UniProt Related Accession: | P35372 |
Molecular Weight: | 20,188 Da |
NCBI Full Name: | Mu-type opioid receptor |
NCBI Synonym Full Names: | opioid receptor, mu 1 |
NCBI Official Symbol: | OPRM1 |
NCBI Official Synonym Symbols: | MOP; MOR; LMOR; MOR1; OPRM; M-OR-1 |
NCBI Protein Information: | mu-type opioid receptor; mu opiate receptor; mu opioid receptor hMOR-1a |
UniProt Protein Name: | Mu-type opioid receptor |
UniProt Synonym Protein Names: | Mu opiate receptor; Mu opioid receptor; MOP; hMOP |
Protein Family: | Mu-type opioid receptor |
UniProt Gene Name: | OPRM1 |
UniProt Entry Name: | OPRM_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |